基于荧光传感器检测食品中赭曲霉毒素A的研究进展

Research progress of detection of ochratoxin A in food based on fluorescence sensor

中文关键词:  荧光传感器  赭曲霉毒素A  纳米材料  荧光染料

英文关键词:fluorescence sensor  ochratoxin A  nanomaterials  fluorescent dye

中文摘要:

      赭曲霉毒素A (ochratoxin A, OTA)是曲霉菌属和青霉菌属产生的一种次级代谢产物, 具有较强的肾毒性、肝毒性、神经毒性和免疫毒性。因其较为稳定的物理化学性质, 使其在食品加工过程中难以被有效去除。OTA广泛存在于谷物、葡萄等多种食品原料及其制品中, 严重威胁人体健康。近年来, 光学传感器作为生命科学领域中的研究热点, 得到了学者们的关注, 其中荧光传感器凭借其简单快捷的优势, 在酶促反应、免疫反应等许多领域得到了广泛应用, 并产生了基于仪器分析、免疫分析学和分子生物学的OTA检测技术。本文基于食品中OTA荧光传感器检测技术发展近况, 综述了荧光检测技术中不同原理、纳米材料和荧光染料在检测中的应用, 从技术分类、应用特点及范围3个角度进行介绍, 为相关农产品中OTA的检测与发展提供参考。

英文摘要:

      Ochratoxin A (ochratoxin A, OTA) is a secondary metabolite produced by Aspergillus and Penicillium. It has strong nephrotoxicity, liver toxicity, neurotoxicity and immunotoxicity. Due to its stable physical and chemical properties, it is difficult to be effectively removed in food processing. OTA is widely present in various food materials such as grains and grapes and other food raw materials, and their products, which seriously threaten human health. In recent years, as a research hotspot in the field of life sciences, optical sensors have attracted the attention of scholars. Among them, fluorescent sensors have been widely used in many fields such as enzymatic reactions and immune reactions due to their simple and fast advantages, and OTA detection technology for instrumental analysis, immune-analysis and molecular biology is produced. Based on the recent development of OTA fluorescence sensor detection technology in food, this article reviewed the application of different technical principles, nanomaterials and fluorescent dyes in fluorescence detection, and introduced them from 3 perspectives: Technical classification, application characteristics and scope, so as to provide reference for OTA detection and development in related agricultural products.