恩诺沙星在余干乌鸡不同组织中的残留消除规律研究

Study on residue elimination rule of enrofloxacin in different tissues of Yugan black-bone fowl

中文关键词:  恩诺沙星  余干乌鸡  残留  休药期

英文关键词:enrofloxacin  Yugan black-bone fowl  residue  withdrawal period

中文摘要:

      目的 研究恩诺沙星可溶性粉在余干乌鸡各组织内的残留消除规律。方法 88只110 d龄健康余干乌鸡, 随机分为11组, 以0.75 g/L饮水连续给药5 d。停药后第0.16、1、3、5、7、9、20、30、40 d采集肌肉、肝脏、肾脏、皮脂样本, 经液相色谱-串联质谱法测定组织中恩诺沙星和环丙沙星残留量, 计算药物代谢半衰期, 并利用WT1.4软件计算恩诺沙星在余干乌鸡体内的休药期。结果 恩诺沙星在余干乌鸡体内的半衰期和休药期均长于普通肉鸡。恩诺沙星可溶性粉在余干乌鸡肌肉、肝脏、肾脏及皮脂中的休药期分别是110、64、67和208 d。结论 为保证乌鸡产品质量安全和消费者健康, 推荐恩诺沙星可溶性粉在余干乌鸡体内的休药期应大于210 d。

英文摘要:

      Objective To study the residue elimination rule of enrofloxacin soluble powder in various tissues of Yugan black-bone fowl. Methods Eighty-eight 110-day-old healthy Yugan black-bone fowls were randomly divided into 11 groups and given 0.75 g/L drinking water continuously for 5 days. Samples of muscle, liver, kidney and sebum were collected at 0.16, 1, 3, 5, 7, 9, 20, 30 and 40 d after drug withdrawal. The residues of enrofloxacin and ciprofloxacin in tissues were determined by liquid chromatography-tandem mass spectrometry. The half-life of drug metabolism was calculated, and the drug withdrawal period of enrofloxacin in Yugan black-bone fowl was calculated by WT1.4 software. Results The half-life and withdrawal period of enrofloxacin in Yugan black-bone fowl were longer than those in common broilers. The withdrawal period of enrofloxacin soluble powder in muscle, liver, kidney and sebum of Yugan black-bone fowl was 110, 64, 67 and 208 d respectively. Conclusion It is recommended that the drug withdrawal period of enrofloxacin soluble powder in Yugan black-bone fowl should be greater than 210 days to ensure the quality and safety of black-bone chicken products and the health of consumers.