滚环等温扩增技术在食源性致病菌检测中的应用

Application of rolling circle amplification technology in the detection of food-borne pathogenic microorganism

中文关键词:  滚环等温扩增技术  食源性致病菌  检测技术

英文关键词:rolling circle amplification technology  food-borne pathogens  detection technology

中文摘要:

      食源性致病菌是影响人类食品安全的重要因素。人类食源性致病菌致病率的逐年上升, 引起世界广泛关注。滚环等温扩增(rolling circle amplification, RCA)技术因具有高特异性、高灵敏度、稳定、操作简单等优点, 在食源性致病菌检测中具有广泛的应用前景。近年来以滚环等温扩增技术为基础, 针对食源性致病菌的检测新技术不断得到发展。本文概述了滚环等温扩增技术的基本原理、技术特点、在食品微生物快速检测方面的应用、存在的不足和解决措施, 指明研究发展新方向, 旨在为食源性致病菌在快速、高通量检测需求上增加新方法, 对食品安全监管具有重要意义。

英文摘要:

      The pathogen of food-borne diseases is an important factor of human food safety. The increasing rate of human disease caused by food-borne pathogenic bacteria has attracted worldwide attention. The rolling circle amplification (RCA) has a wide application prospect in the detection of the pathogen of food-borne diseases due to its high specificity, high sensitivity, stability, simple operation. In recent years, the new detection technology based on RCA for the pathogen of food-borne diseases has been developed continuously. This review described the basic principle and characteristics of RCA, and its application, the existing shortcomings and solutions in rapid detecting food microorganism, pointed out the new direction of research and development, so as to add new methods for rapid detection and high throughput for food-borne pathogenic, which is of great significance for food safety supervision.