金花茶不同部位化学成分差异分析

Analysis of the differences in chemical components from different parts of Camellia nitidissima C. W. Chi

中文关键词:  金花茶  超高效液相色谱法  指纹图谱  化学成分  药食同源

英文关键词:Camellia nitidissima C. W. Chi  ultra performance liquid chromatography  fingerprint  chemical composition  medicine and food homology

中文摘要:

      目的 比较防普、越南、大叶金花茶叶、花两个部位化学指纹图谱差异, 分析其化学成分类型及部分有效成分含量差异, 为合理开发和利用金花茶提供依据。方法 采用超高效液相色谱法(ultra performance liquid chromatography, UPLC)建立防普、越南、大叶金花茶叶和花的化学指纹图谱; 利用中药色谱指纹图谱相似度评价系统(2012版)计算不同部位共有峰和相似度, 并分析各部位特征峰; 利用SPSS 23.0, 采用成对样本t检验, 对叶和花间不同化学成分含量差异进行比较。结果 在320和210 nm波长下, 防普金花茶叶和花的相似度分别为0.504、0.054; 越南金花茶叶和花的相似度分别为0.151、0.478; 大叶金花茶叶和花的相似度分别为0.069、0.748, 3个品种金花茶的叶和花相似度均较低。防普金花茶花中金丝桃苷、叶中二氢槲皮素含量最高且花和叶中绿原酸、二氢槲皮素、水杨酸、芹菜素含量差异达到显著水平(P<0.05); 越南金花茶花中牡荆素、叶中水杨酸含量最高, 且叶和花中绿原酸、牡荆素、水杨酸、芹菜素含量差异达到显著水平(P<0.05); 大叶金花茶花中牡荆素、叶中二氢槲皮素含量最高, 且花和叶中没食子酸、表儿茶素含量差异达到显著水平(P<0.05)。结论 3个品种金花茶叶和花化学成分和含量均存在显著差异, 叶和花在药理活性和功效上各有侧重, 药用和食用过程中应根据实际情况筛选使用。

英文摘要:

      Objective To compare the chemical fingerprints of the leaves and flowers of Camellia niridissima Chi, Camellia insularis Orel et Curry and Camellia chrysantha (Hu) Tuyama var. macrophylla S. L. Mo et S. Z. Huang, and analyze the differences of chemical components and the content of some effective ingredients between the types, so as to provide a basis for rational development and utilization of Camellia nitidissima C. W. Chi. Methods Ultra performance liquid chromatography (UPLC) was used to establish leaves and flowers chemical fingerprints of Camellia niridissima Chi, Camellia insularis Orel et Curry and Camellia chrysantha (Hu) Tuyama var. macrophylla S. L. Mo et S. Z. Huang, the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2012) was run to calculate the common peaks and similarities of different parts, and analyzed the characteristic peaks of each part; SPSS 23.0 was run to compare the differences in component content of the leaves and flowers using the paired sample t-test. Results At 320 and 210 nm wavelengths, the similarity of the leaves and flowers of Camellia niridissima Chi was 0.504 and 0.054; the similarity of the leaves and flowers of Vietnam Camellia insularis Orel et Curry was 0.151 and 0.478; the similarity of the leaves and flowers of Camellia chrysantha (Hu) Tuyama var. macrophylla S. L. Mo et S. Z. Huang was 0.069 and 0.748, the similarities of leaves and flowers of the 3 varieties of them was low. The content of hyperin and dihydroquercetin in the flowers of Camellia niridissima Chi was the highest, and the content of chlorogenic acid, dihydroquercetin, and salicylic acid in the flowers and leaves were significantly different (P<0.05); the content of vitexin and salicylic acid in leaves of Camellia insularis Orel et Curry were the highest, and the content of chlorogenic acid, vitexin, salicylic acid and apigenin in leaves and flowers were significantly different (P<0.05); the content of vitexin and dihydroquercetin in flower of Camellia chrysantha (Hu) Tuyama var. macrophylla S. L. Mo et S. Z. Huang was the highest, and the content of gallic acid and epicatechin in flowers and leaves were significantly different (P<0.05). Conclusion There are significant differences in the chemical composition and content of the 3 varieties of Camellia nitidissima C. W. Chi, the leaves and flowers have their own emphasis on pharmacological activity and efficacy, and should be screened and used according to actual conditions in the process of medicinal and edible use.