气相色谱-离子迁移谱法结合多元统计分析方法鉴定不同产地贝母

Identification of Fritillariae from different origins based on gas chromatography-ion mobility spectrometry combined with multivariate statistical analysis

中文关键词:  气相色谱-离子迁移谱法  挥发性物质  贝母  主成分分析

英文关键词:gas chromatography-ion mobility spectrometry  volatile components  Fritillaria  principal component analysis

中文摘要:

      目的 比较13种不同产地贝母中的挥发性物质组成差异, 建立一种简便、快速、准确鉴别不同产地贝母的特征指纹图谱的检测方法。方法 气相色谱-离子迁移谱法(gas chromatography-ion mobility spectrometry, GC-IMS)对不同产地贝母的挥发性物质进行定性和相对定量分析, 结合主成分分析(principal component analysis, PCA)法明确其挥发性成分的差异。结果 GC-IMS指纹图谱显示不同产地的贝母挥发性物质组成存在明显差异, 对42种挥发性成分进行定性分析, 包括单体及部分物质的二聚体, 瓦布贝母中特有挥发性成分为甲基乙基甲酮、戊醛、2-庚酮、2-正戊基呋喃等; 太白贝母中特有挥发性成分为丁酸内酯、2,3-二乙基-6-甲基吡嗪、2,6-二甲基吡嗪; 浙贝母中的特有挥发性成分主要为醛类, 如: 苯乙醛、庚醛、辛醛、戊二醛和糠醛; 伊贝母中特有挥发性成分为乙偶姻和1-戊醇; 阿坝松贝中的正壬醛; 理塘贝母中的己基甲基酮、正己醛、3-甲基丁醛和1-己醇; 甘肃贝母中的愈创木酚; 西藏贝母中的乙醇等。PCA分析将13种贝母分为5大类: 川贝母类、浙贝母、伊贝母、瓦布贝母及太白贝母, 各组样品之间聚类差异明显。结论 GC-IMS技术结合PCA方法证明不同产地的贝母挥发性成分存在显著差异, 为贝母作为保健食品原材料的质量控制及产地鉴别的研究提供参考。

英文摘要:

      Objective To compare the composition of volatile substances in Fritillaria from 13 kinds of different habitats, and establish a simple, rapid and accurate fingerprint detection method to identify the characteristic fingerprints of Fritillaria from different origins. Methods The volatile components of 13 kinds of Fritillaria from different origins were analyzed qualitatively and quantitatively by gas chromatography-ion mobility spectrometry (GC-IMS), and coupled with principal component analysis (PCA), the differences of volatile components were determined. Results GC-IMS fingerprints showed that there were significant differences in the composition of volatile substances of Fritillaria from different origins, qualitative analysis was performed on 42 kinds of volatile components, including monomers and dimers of some substances, the unique volatile components in Fritillaria wabuensis were methyl ethyl ketone, pentanal, 2-heptanone, 2-pentylfura, etc.; the unique volatile components in Fritillaria taipaiensis were gamma-butyrolactone, 2,3-diethyl-6-methylpyrazine and 2,6-dimethylpyrazine; the unique volatile components in Fritillaria thunbergii were mainly aldehydes such as phenylacetaldehyde, heptaldehyde, octanal, pentanal and furfural; the unique volatile components in Fritillaria pallidiflora Schrek were acetoin and 1-pentanol; the unique volatile components in Fritillaria Aba Songpan and Fritillaria Litang were n-nonanal and ethanol, respectively. Thirteen species of Fritillaria could be divided into 5 categories successfully through PCA analysis, including Fritillaria cirrhosa, Fritillaria thunbergii, Fritillaria pallidiflora Schrek, Fritillaria wabuensis and Fritillaria taipaiensis, the clustering difference among each group was obvious. Conclusion GC-IMS coupled with PCA proves that there are significant differences in the component of volatile substances of Fritillaria from different origins, which provides a reference for the quality control and origin identification of Fritillaria as a raw material of health food.