鼠李糖乳杆菌R9639对小鼠免疫功能的影响及机制研究

Effects of Lactobacillus rhamnosus R9639 on immune function in mice and its mechanism

中文关键词:  鼠李糖乳杆菌R9639  小鼠  免疫功能  剂量

英文关键词:Lactobacillus rhamnosus R9639  mouse  immunomodulatory effect  dose

中文摘要:

      目的 研究鼠李糖乳杆菌(Lactobacillus rhamnosus) R9639对小鼠免疫功能的调控作用。方法 分别灌胃给予小鼠2.5、5.0、15.0 mg/kg的鼠李糖乳杆菌R9639 30 d后, 测定小鼠脏器/体重比; 抗体生成细胞实验和血清溶血素测定实验评价其对小鼠体液免疫功能的影响; 脾淋巴细胞转化实验和迟发型变态反应实验评价其对小鼠细胞免疫功能的影响; 小鼠碳廓清实验和小鼠腹腔巨噬细胞吞噬鸡红细胞实验评价其对小鼠单核-巨噬细胞功能的影响; 自然杀伤(natural killer, NK)细胞活性测定实验评价其对小鼠NK细胞活性的影响。 结果 鼠李糖乳杆菌R9639对小鼠胸腺/体重比值、脾脏/体重比值均无显著影响(P>0.05)。与对照组相比, 5.0、15.0 mg/kg的鼠李糖乳杆菌R9639组半数溶血素值(half value of hemolysin, HC50)明显升高(P<0.05)、迟发型变态反应显著增强(P<0.05)、小鼠的碳廓清能力显著提高(P<0.05); 15.0 mg/kg的鼠李糖乳杆菌R9639组小鼠NK细胞活性显著增强(P<0.05)。结论 鼠李糖乳杆菌R9639可通过增强体液免疫、细胞免疫、单核-巨噬细胞功能及NK细胞活性来增强小鼠的免疫功能。

英文摘要:

      Objective To study the regulatory effects of Lactobacillus rhamnosus R9639 on immune function in mice. Methods The mice were treated with Lactobacillus rhamnosus R9639 at doses of 2.5, 5.0 and 15.0 mg/kg, respectively, by gavage for 30 days, and then the organ/body weight ratio was measured; the antibody-producing cell assay and serum hemolysin assay were conducted to evaluate their effects on the humoral immune function of mice; spleen lymphocyte transformation test and delayed allergic reaction test were used to evaluate their effects on cellular immune function of mice; the carbon clearance test of mice and the phagocytosis of chicken red blood cells by peritoneal macrophages of mice were used to evaluate the effects on the function of mononuclear-macrophage cells in mice; and natural killer (NK) cell activity assay was used to evaluate its effect on NK cell activity in mice. Results Lactobacillus rhamnosus R9639 had no significant effect on the thymus/body weight ratio and spleen/body weight ratio of mice (P>0.05). Compared with the control group, the half value of hemolysin (HC50) was significantly increased (P<0.05) and the delayed-type allergic reaction was significantly enhanced (P<0.05) in the 5.0 and 15.0 mg/kg Lactobacillus rhamnosus R9639 groups, as well as the carbon clearance of mice (P<0.05); and the NK cell activity of mice in the Lactobacillus rhamnosus R9639 group at 15.0 mg/kg was significantly increased (P<0.05). Conclusion Lactobacillus rhamnosus R9639 can enhance the immune function of mice by enhancing humoral immunity, cellular immunity, monocyte-macrophage function and NK cell activity.