高效液相色谱-电感耦合等离子体质谱联用法分析新疆巴旦木中硒形态

Analysis of selenium species in Xinjiang Amygdalus communis L. by high performance liquid chromatography-inductively coupled plasma mass spectrometry

中文关键词:  高效液相色谱-电感耦合等离子体质谱联用法  巴旦木  硒形态  阴离子分析柱

英文关键词:high performance liquid chromatography-inductively coupled plasma mass spectrometry  Amygdalus communis L.  selenium species  anion analytical column

中文摘要:

      目的 建立高效液相色谱-电感耦合等离子体质谱联用法(high performance liquid chromatography-inductively coupled plasma mass spectrometry, HPLC-ICP-MS)测定新疆巴旦木(Amygdalus communis L.)中硒代蛋氨酸(selenomethionine, SeMet)和亚硒酸根[selenous acid, Se(IV)] 2种硒形态的分析方法。方法 研究采用超声波辅助酶提法提取巴旦木样品中硒形态, 选用Dionex IonPac AS19阴离子分析柱进行分离, pH为9.2的磷酸二氢铵水溶液作为流动相, 进样量为100 μL。结果 实现了在500 s内2种硒形态基线分离, SeMet和Se(IV)的方法检出限均为0.006 mg/kg, 加标回收率为90%~106%。对巴旦木样品中SeMet和Se(IV)含量进行测定, 2种硒形态含量之和占总硒的75.8%~81.2%。结论 所建立的方法易操作、分离效果好且检出限低, 可以作为一种分析巴旦木中2种硒形态的有效方法。

英文摘要:

      Objective To establish an analytical method for the determination of selenomethionine (SeMet) and selenous acid [Se(IV)] forms in Xinjiang Amygdalus communis L. by high performance liquid chromatography-inductively coupled plasma mass spectrometry. Methods In this study, the form of selenium in almond samples was extracted by ultrasonic-assisted enzyme method, Dionex IonPac AS19 anion exchange column was used for separation, ammonium dihydrogen phosphate aqueous solution with pH 9.2 was used as the mobile phase, and the injection amount was 100 μL. Results Baseline separation of the 2 kinds of selenium forms within 500 s was achieved with the detection limits of both SeMet and Se(IV) being 0.006 mg/kg, and the spiked recoveries being 90%?106%, and the proportion of SeMet and Se(IV) in the total selenium were 75.8%?81.2%. Conclusion The established method is easy to operate, has good separation effect and low detection limit, and can be used as an effective method for analyzing 2 kinds of selenium species in Amygdalus communis L.