乙醇/磷酸氢二钾双水相同步萃取脐橙果皮中黄酮和果胶

Simultaneous extraction of flavonoids and pectin from navel orange peel by ethanol/dipotassium hydrogen phosphate aqueous two-phase system

中文关键词:  双水相萃取  响应面分析  黄酮类化合物  果胶

英文关键词:aqueous two-phase system  response surface experiments  flavonoids  pectin

中文摘要:

      目的 采用乙醇/磷酸氢二钾双水相体系同步分离脐橙皮中的黄酮和果胶。方法 以脐橙果皮为原料, 通过单因素实验和响应面实验对双水相萃取条件进行优化, 分析了双水相体系乙醇体积分数、磷酸氢二钾质量浓度、萃取温度对黄酮和果胶提取率的影响。结果 乙醇/磷酸氢二钾双水相体系能同时分离脐橙果皮中的黄酮和果胶, 其中上相为黄酮类化合物, 下相经脱盐、热水浸提和乙醇沉淀后获得果胶。最佳提取条件为: 乙醇体积分数35%, 磷酸氢二钾质量分数为18%, 萃取温度为78 ℃, 此条件下的果胶得率为(8.35±0.09)%, 黄酮得率为(3.32±0.08)%。结论 本研究建立了乙醇/磷酸氢二钾双水相体系同步分离脐橙果皮中黄酮和果胶的方法, 具有工艺简单、提取率高的优点, 为脐橙果皮的综合利用和开发提供了可供参考的思路和技术手段。

英文摘要:

      Objective To separate the flavonoids and pectin of navel orange peel by ethanol/dipotassium hydrogen phosphate aqueous two-phase system synchronously. Methods The aqueous two-phase system conditions were optimized by single-factor experiment and response surface experiment, and the effects of ethanol volume fraction, dipotassium hydrogen phosphate mass fraction and extraction temperature on the extraction ratea of flavonoids and pectin were studied. Results The flavonoids and the pectin could be separated by the ethanol/dipotassium hydrogen phosphate aqueous two-phase system, the upper phase was flavonoids, while the lower phase was pectin after desalting, hot water extraction and ethanol precipitation. The optimal extraction conditions were as follows: Ethanol volume fraction of 35%, dipotassium hydrogen phosphate mass fraction of 18%, and the extraction temperature of 78 ℃, under these conditions, the extraction yield of the pectin and the flavonoids were (8.35±0.09)% and (3.32±0.08)%, respectively. Conclusion The ethanol/dipotassium hydrogen phosphate aqueous two-phase system can simultaneously separate flavonoids and pectin from navel orange peel, which is characterized with the advantages of simple separation process and high yield, which provides reference ideas and technical means for the comprehensive utilization and development of navel orange peel.