基于87Sr/86Sr和δ13C对进口大麦进行产地溯源

Origin traceability of imported barley based on 87Sr/86Sr and δ13C

中文关键词:  87Sr/86Sr  δ13C  进口大麦  产地溯源  稳定同位素比质谱法

英文关键词:87Sr/86Sr  δ13C  imported barley  origin traceability  stable isotope ratio mass spectrometry

中文摘要:

      目的 建立基于87Sr/86Sr和δ13C稳定同位素比质谱法(stable isotope ratio mass spectrometry, IRMS)的进口大麦产地溯源技术。方法 样品干燥粉碎后, 经硝酸消解, 锶特效树脂净化后利用热电离质谱法(thermal ionization mass spectrometry, TIMS)分析检测87Sr/86Sr; 样品用锡囊包裹后, 利用元素分析-稳定同位素比质谱法分析检测δ13C; 利用SPSS 25.0软件对进口大麦的87Sr/86Sr和δ13C进行正态性验证、方差分析、事后多重比较分析和判别分析。结果 不同进口国大麦中的87Sr/86Sr和δ13C具有显著性差异, 经判别分析, 进口自澳大利亚、法国和美国的大麦可以达到100%正确判别率, 整体正确判别率达到86.2%, 若将美国和加拿大进口的大麦归类为北美洲进口大麦, 则可实现进口自北美、法国和澳大利亚大麦的100%正确判别率。结论 利用87Sr/86Sr和δ13C可以对不同进口国大麦进行产地溯源分析。

英文摘要:

      Objective To establish the origin traceability technology of imported barley based on 87Sr/86Sr and δ13C stable isotope ratio mass spectrometry. Methods The samples were dried and crushed, digested with nitric acid, purified with strontium-specific resin, and then 87Sr/86Sr was analyzed and detected by thermal ionization mass spectrometry (TIMS); after the samples were wrapped with tin capsules, δ13C was detected by elemental analysis–stable isotope ratio mass spectrometry (IRMS); the SPSS 25.0 software was used for normality verification, variance analysis, post multiple comparison analysis and discriminant analysis of 87Sr/86Sr and δ13C of imported barley. Results The 87Sr/86Sr and δ13C values of barley from different importing countries showed significant differences, the barley imported from Australia, France and the United States could achieve 100% correct discrimination rate and the overall correct discrimination rate reached 86.2%, if the barley imported from the United States and Canada was classified as North American imported barley, the 100% correct discrimination rate of barley imported from North America, France and Australia could be achieved. Conclusion The origin traceability of barley from different importing countries can be analyzed using 87Sr/86Sr and δ13C.