多壁碳纳米管QuEChERS-液相色谱-串联质谱法快速检测茶叶中氯虫苯甲酰胺残留量

Rapid determination of chlorantraniliprole in tea by multi-walled carbon nanotubes QuEChERS-liquid chromatography-tandem mass spectrometry

中文关键词:  液相色谱-串联质谱法  茶叶  氯虫苯甲酰胺  QuEChERS  多壁碳纳米管

英文关键词:liquid chromatography-tandem mass spectrometry  tea  chlorantraniliprole  QuEChERS  multi-walled carbon nanotubes

中文摘要:

      目的 建立多壁碳纳米管QuEChERS-液相色谱-串联质谱法快速测定茶叶中氯虫苯甲酰胺残留量的分析方法。方法 样品经乙腈提取, 多壁碳纳米管QuEChERS方法净化, 经Agilent Eclipse Pluse C18色谱柱(2.1 mm×50 mm, 1.8 μm)分离, 以0.1%甲酸水溶液(A)-乙腈(B)为流动相进行梯度洗脱, 并在串联质谱正离子多反应监测(multiple reaction monitoring, MRM)模式下进行测定, 空白基质外标法定量。结果 5种茶叶中氯虫苯甲酰胺在0.1~50.0 ng/mL范围内线性良好(r>0.995), 检出限为0.5 μg/kg, 定量限为1.2 μg/kg, 在0.5、5.0、50.0 μg/kg添加水平下, 平均加标回收率在84.4%~103.9%之间, 相对标准偏差为0.8%~8.3% (n=6)。结论 该方法具有较高的灵敏度, 操作简便、准确可靠, 适用于茶叶中氯虫苯甲酰胺农药残留量的检测。

英文摘要:

      Objective To establish a method for the rapid determination of chlorantraniliprole in tea by multi-walled carbon nanotubes QuEChERS-liquid chromatography-tandem mass spectrometry. Methods The samples were extracted by acetonitrile solution, purified by multi-walled carbon nanotubes QuEChERS method, separated by Agilent Eclipse Pluse C18 column (2.1 mm×50 mm, 1.8 μm) by gradient elution using 0.1% formic acid solution (A)-acetonitrile (B) as mobile phase, detected on a triple quadrupole tandem mass spectrometry with electrospray ionization source operating in positive ion mode under multiple reaction monitoring (MRM) mode, and quantified by matrix external standard method. Results The linearities of chlorantraniliprole in 5 kinds of tea were good in the range of 0.1-50.0 ng/mL (r>0.995), the limits of detection were 0.5 μg/kg, the limits of quantitation were 1.2 μg/kg, the average recoveries at 0.5, 5.0, 50.0 μg/kg addition levels were ranged from 84.4% to 103.9%, and the relative standard derivations were within 0.8%~8.3% (n=6). Conclusion The method is sensitive, easy to operate, accurate and reliable, which is suitable for the determination of chlorantraniliprole residues in tea.