酶水解-阴离子色谱法联用测定婴幼儿配方奶粉中果聚糖的总含量及平均聚合度

Determination of total content and average degree of polymerization of fructan in infant formula milk powder by enzyme hydrolysis-anion chromatography

中文关键词:  婴幼儿配方奶粉  酶水解-阴离子色谱法  果聚糖  平均聚合度

英文关键词:infant formula milk powder  enzyme hydrolysis-anion chromatography  fructan  average degree of polymerization

中文摘要:

      目的 建立酶水解-阴离子色谱法测定婴幼儿配方奶粉中果聚糖总含量和平均聚合度的分析方法。 方法 通过酶水解将样品中的果聚糖水解成果糖、葡萄糖, 以PA1阴离子交换柱分离, 脉冲积分安培检测器检测果聚糖总含量, 并以果糖与葡萄糖产生的比例计算平均聚合度。结果 果糖、葡萄糖在0.25~25.00 mg/L范围内线性关系良好(r2>0.999), 果聚糖的回收率为96.7%~104.1%, 方法检出限为195 mg/kg, 定量限为650 mg/kg。实际样品测定中, 日间的相对标准偏差为1.15%~3.87% (n=6)。结论 此方法灵敏度和精密度较好, 可用于婴幼儿配方奶粉中果聚糖的总含量及平均聚合度的测定。

英文摘要:

      Objective To establish an analytical method for the determination of the total content and average polymerization degree of fructan in infant formula milk powder by enzymatic hydrolysis-anion chromatography. Methods The fructan in the sample was hydrolyzed by enzyme to obtain fructose and glucose, which were separated by PA1 anion exchange column, the total content of fructan was detected by pulse integral amperometric detector, and the average degree of polymerization was calculated based on the ratio of fructose to glucose. Results The linear relationship between fructose and glucose were good within the range of 0.25?25.00 mg/L (r2>0.999), the recovery of fructan was 96.7%?104.1%, and the limit of detection of the method was 195 mg/kg and the limit of quantitation was 650 mg/kg. In the actual sample determination, the relative standard deviations during daytime were 1.15%?3.87% (n=6). Conclusion This method has good sensitivity and precision, and can be used to determine the total content and average polymerization degree of fructan in infant formula milk powder.