东紫苏多酚提取物对HepG2细胞氧化损伤的保护作用研究

Study on the protective effect of polyphenol extract from Elsholtzia bodinieri Vaniot against oxidative damage of HepG2 Cells

中文关键词:  东紫苏多酚  抗氧化  HepG2细胞  氧化应激损伤  保护作用

英文关键词:Elsholtzia bodinieri Vaniot polyphenols  antioxidant  HepG2 cells  oxidative stress damage  protection effect

中文摘要:

      目的 研究东紫苏(Elsholtzia bodinieri Vaniot)多酚提取物对H2O2诱导的HepG2细胞氧化应激损伤的保护作用。方法 采用福林酚(Folin-Ciocalte)法测定东紫苏提取物的多酚含量, 通过测定1,1-二苯基-2-苦基肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基清除能力(DPPH radical scavenging activity, DRSA)、铁离子还原/抗氧化能力(ferric reducing antioxidant power, FRAP)、过氧化氢清除活性(hydrogen peroxide scavenging activity, HPSA)和总抗氧化能力(trolox equivalent antioxidant capacity, TEAC)来评价东紫苏多酚体外抗氧化活性, 用噻唑蓝法(thiazolyl blue tetrazolium bromide, MTT)测定不同浓度的东紫苏多酚对HepG2细胞的毒性作用, 并确定3个无毒性作用浓度, 研究其对HepG2细胞增殖抑制作用, 以800 μmol/L的H2O2诱导HepG2细胞建立氧化应激损伤模型, 通过测定HepG2细胞中活性氧(reactive oxygen species, ROS)含量、抗氧化酶[超氧化物歧化酶(superoxide dismutase, SOD)、过氧化氢酶(catalase, CAT)]活力、谷胱甘肽(glutathione, GSH)含量以及丙二醛(malonicdialdehyde, MDA)含量的变化情况, 研究在5.00、2.50和1.25 μg/mL无毒性作用浓度下东紫苏多酚对HepG2细胞氧化损伤的保护作用。结果 东紫苏多酚含量为(30.91±1.33) μmol/g DW, DRSA值为(17.45± 0.54) μmol/g DW, FRAP值为(52.64±0.05) μmol/g DW, HPSA值为(27.12±0.17) μmol/g DW, TEAC值为(186.45±0.16) μmol/g DW。东紫苏多酚提取物能使受氧化损伤的HepG2细胞内ROS和MDA含量明显减少, 并且能提高受氧化损伤细胞内GSH含量以及SOD和CAT活力。结论 东紫苏多酚提取物具有较好的体外抗氧化活性, 对HepG2细胞氧化应激损伤具有一定的保护作用。

英文摘要:

      Objective To study the protective effect of polyphenol extract of Elsholtzia bodinieri Vaniot on oxidative stress injury of HepG2 cells induced by H2O2. Methods The total polyphenol content was determined by Folin-Ciocalte method. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (DRSA), ferric reducing antioxidant power (FRAP), hydrogen peroxide scavenging activity (HPSA) and total antioxidant capacity (TEAC) were measured to evaluate the in vitro antioxidant activity of polyphenols. Thiazolyl blue tetrazolium bromide (MTT) method was used to determine the toxic effects of different concentrations of polyphenols on HepG2 cells, and 3 nontoxic concentrations were determined to study their inhibitory effects on the proliferation of HepG2 cells. HepG2 cells were induced with 800 μmol/L H2O2 to establish an oxidative stress damage model. The content of reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH) and the activities of superoxide dismutase (SOD), catalase (CAT) in HepG2 cells were measured in order to study the protective effect of polyphenol extract from Elsholtzia bodinieri Vaniot oxidative damage of HepG2 cells in non-toxic concentration of 5.00, 2.50 and 1.25 μg/mL. Results The total polyphenol content was (30.91±1.33) μmol/g DW, DRSA value was (17.45± 0.54) μmol/g DW, FRAP value was (52.64±0.05) μmol/g DW, HPSA value was (27.12±0.17) μmol/g DW and TEAC value was (186.45±0.16) μmol/g DW respectively. The polyphenols from Elsholtzia bodinieri Vaniot reduced ROS and MDA content, and increased GSH content, SOD and CAT activity in oxidative damage HepG2 cells. Conclusion The polyphenol extract from Elsholtzia bodinieri Vaniot has good antioxidant activity in vitro, and provides a certain protective effect on oxidative stress damage of HepG2 cells.