核酸检测技术检测肉类种源研究进展

Research progress on detection of meat provenance by nucleic acid detection technology

中文关键词:  核酸检测  掺假  肉制品  聚合酶链式反应  梯型熔解温度等温扩增技术

英文关键词:nucleic acid testing  adulteration  meat products  polymerase chain reaction  ladder-shape melting temperature isothermal amplification

中文摘要:

      肉及肉制品是人类重要的食物来源, 为人体提供必要的营养素。但是以经济为目的的肉制品掺假, 是食品安全中屡禁不止的全球性问题。快速、准确、有效的检测技术是有效监督肉类掺假的关键。本文综述了核酸检测技术中热循环扩增技术(如普通PCR、实时PCR、多重PCR)和等温扩增技术[如环介导等温扩增(loop-mediated isothermal amplification, LAMP)技术、重组酶聚合酶扩增(recombinase polymerase amplification, RPA)技术、滚环扩增(rolling circle amplification, RCA)技术、交叉引物等温扩增(cross prime amplification, CPA)技术等]的原理及在肉类种源鉴别中的应用。提出梯型熔解温度等温扩增(ladder-shape melting temperature isothermal amplification, LMTIA)技术, 以期推进核酸检测技术的研究及在肉制品领域的应用。在肉类种源的检测中, 实时荧光定量PCR技术、跨越式滚环等温扩增(saltatory rolling circle amplification, SRCA)技术等均能检出0.01%的掺伪, 可用于定量检测, 表明这些核酸技术在肉类种源检测中有很好的应用前景。

英文摘要:

      Meat and meat products are important food sources for human beings and provide necessary nutrients for human body. However, adulteration of meat products for economic purposes is a global problem that has been banned repeatedly in food safety. Fast, accurate and effective detection technologies are the key to effectively supervise meat adulteration. This paper reviewed the thermal cycle amplification technologies in nucleic acid detection technologies [such as ordinary polymerase chain reaction (PCR), real-time PCR, multiplex PCR] and isothermal amplification technologies [such as loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), rolling circle amplification (RCA), cross prime amplification (CPA)] and their application in the field of meat products were discussed in the paper, ladder-shape melting temperature isothermal amplification (LMTIA) was proposed. which would help us to reveal the adulteration of meat products from the perspective of nucleic acid detection, and promote the research and application of nucleic acid detection technology in the field of meat products. In the detection of meat adulteration, 0.01% adulteration can be detected by real-time fluorescence quantitative PCR and saltatory rolling circle amplification (SRCA), which can be used for quantitative detection, indicating that these nucleic acid technologies have a good application prospect in the detection of meat provenance.