用于亚硒酸钠检测的近红外荧光探针的设计合成及其应用研究

Design, synthesis and application of a near infrared fluorescent probe for the detection of sodium selenite

中文关键词:  荧光探针  亚硒酸钠  乙酰丙酸基  近红外

英文关键词:fluorescent probe  sodium selenite  levulinyl group  near infrared

中文摘要:

      目的 建立一种基于近红外荧光探针的亚硒酸钠检测方法。方法 基于荧光探针的构建方法, 在近红外荧光团中引入乙酰丙氨酸基对荧光团结构中羟基进行保护并淬灭荧光, 设计并合成荧光增强型近红外探针SeP1, 通过核磁氢谱(1hydrogen-nuclear magnetic resonance, 1H NMR)、核磁碳谱(13carbon-nuclear magnetic resonance, 13C NMR)和高分辨质谱法(high resolution mass spectrometry, HRMS)的确证其化学结构, 通过荧光光谱法研究探针SeP1对亚硒酸钠的识别效果。结果 在多种分析物中, 探针SeP1对亚硒酸钠具有高效专一的荧光选择性, 其最大荧光发射在近红外区(657 nm), 能够克服基质中常见背景荧光干扰。在其他常见阴离子存在的情况下, 具有较强的抗干扰能力并能够对亚硒酸钠实现裸眼识别。该探针对亚硒酸钠的最低检测限为0.068 μmol/L。其原理是: 当亚硒酸根离子与探针结构中乙酰丙基基团发生特异性反应后, 使得探针结构中羟基脱保护重新生成带有羟基的中间体1, 释放出强烈的荧光, 通过高分辨质谱进一步确证了上述识别机制。 结论 该探针对亚硒酸钠具有高效专一的识别能力, 并成功的应用于饮用水中亚硒酸钠加标回收检测, 具备较强的实用性。

英文摘要:

      Objective To develop a method for the determination of Na2SeO3 based on fluorescent probe. Methods Based on the construction method of fluorescent probe, a fluorescence enhanced near-infrared probe SeP1 was designed by introducing levulinyl group into the near-infrared fluorophore to protect the hydroxyl and quench the fluorescence, and its chemical structure was confirmed by 1hydrogen-nuclear magnetic resonance (1H NMR), 13carbon-nuclear magnetic resonance (13C NMR) and high resolution mass spectrometry (HRMS), the recognition effect of probe SeP1 on Na2SeO3 was studied by fluorescence spectrometry. Results In a variety of analytical species, the probe SeP1 had high efficiency and specific near-infrared fluorescence selectivity for Na2SeO3, and its maximum fluorescence emission was in the near infrared region (657 nm), which could overcome the common background fluorescence interference in the matrix. In the presence of other common anions, it had strong anti-interference ability, and could realize naked eye recognition for Na2SeO3. The detection limit of the probe for Na2SeO3 was 0.068 μmol/L. The recognition mechanism was: When the selenite ion reacted specifically with the acetyl group in the probe structure, the hydroxyl group in the probe structure was deprotected, and intermediate 1 with hydroxyl structure was regenerated, releasing strong fluorescence, the above recognition mechanism was further confirmed by high-resolution mass spectrometry (HRMS). Conclusion The probe showed high efficiency and specificity in detecting Na2SeO3, and it has been successfully applied to the detection of Na2SeO3 in drinking water with high practicability.