白芷粗多糖的提取工艺优化及辐照对其含量和活性的影响

Optimization of extraction technology of crude polysaccharides from Angelica dahurica and effects of irradiation on its content and activity

中文关键词:  白芷粗多糖  提取优化  高能电子束  抗氧化活性  抑菌活性

英文关键词:crude polysaccharides of Angelica dahurica  optimization of extraction  high energy electron beam  antioxidant activity  antibacterial activity

中文摘要:

      目的 优化白芷粗多糖的提取工艺, 并研究高能电子束辐照对其含量、抗氧化活性和抑菌活性的影响。方法 在单因素的实验基础上进行三因素三水平的Box-Benhnken中心组合实验, 得到白芷粗多糖最优水提醇沉工艺; 紫外-可见分光光度法测定经不同剂量高能电子束辐照后白芷粗多糖含量和清除羟自由基(·OH)、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基的能力; 菌饼法测定各辐照剂量处理下白芷粗多糖对金黄色葡萄球菌、大肠杆菌和希瓦氏菌的抑制能力。结果 白芷粗多糖的最优水浴提取工艺为: 温度100 ℃、时间3 h、液料比60 (mL/g), 此条件下, 粗多糖提取率可达到8.02%。高能电子束辐照剂量为0、1、3 kGy时, 白芷粗多糖含量差异不显著(P>0.05); 5~15 kGy辐照组含量差异显著(P<0.05), 随着辐照剂量的增加含量上升, 最高可达到17.62%。辐照会明显降低白芷粗多糖对·OH、DPPH自由基的清除能力, 且不同辐照剂量下的白芷粗多糖的清除能力差异显著(P<0.05)。5、7 kGy辐照会升高白芷粗多糖对菌落的抑制能力, 其余剂量辐照会降低其抑菌活性。结论 高能电子束可以用于白芷辐照加工, 为不影响粗多糖含量与活性, 辐照剂量应为5 kGy。

英文摘要:

      Objective To optimize the extraction technology of crude polysaccharides from Angelica dahurica and to study the effects of high energy electron beam irradiation on its content, antioxidant activity and antimicrobial activity. Methods The Box-Benhnken central combination test with three factors and three levels was carried out on the basis of single factor test to obtain the optimal water extraction and alcohol precipitation process of crude polysaccharides of Angelica dahurica. The content of crude polysaccharides and scavenging ability of ·OH and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals of Angelica dahurica irradiated with different doses of high energy electron beam were determined by ultraviolet visible spectrophotometry. Bacterial cake method was used to determine the inhibition ability of crude polysaccharides of Angelica dahurica against Staphylococcus aureus, Escherichia coli and Shivella under different irradiation doses. Results The optimal water bath extraction conditions were: temperature 100 ℃, time 3 h and liquid material ratio 60 (mL/g). Under these conditions, the extraction yield of crude polysaccharides could reach 8.02%. When the irradiation dose of high energy electron beam was 0, 1, 3 kGy, the crude polysaccharide content of Angelica dahurica had no significant difference (P>0.05). The content of 5-15 kGy radiation group was significantly different (P<0.05), and increased with the increase of radiation dose, reaching the highest of 17.62%. Irradiation significantly reduced the scavenging ability of ·OH and DPPH free radicals of the crude polysaccharides of Angelica dahurica, and the scavenging ability of the crude polysaccharides of Angelica dahurica at different irradiation doses was significantly different (P<0.05). Irradiated with 5 and 7 kGy, the inhibition ability of crude polysaccharides of Angelica dahurica to the colony was increased, and the other doses of irradiation would reduce its antibacterial activity. Conclusion High energy electron beam can be used in the irradiation process of Angelica dahurica, in order not to affect the content and activity of crude polysaccharide, the irradiation dose should be 5 kGy.