蛋白质免疫印迹技术在水产品中的应用

Application of western blotting in aquatic products

中文关键词:  蛋白质免疫印迹技术  水产品  蛋白质组学  质量与安全

英文关键词:western blotting  aquatic products  proteomics  quality and safety

中文摘要:

      蛋白质免疫印迹技术作为一门新兴的方法, 在蛋白质组学研究中得到广泛应用。该技术可对待测样品中目的蛋白质进行定性和半定量分析, 为探究水产品中蛋白质组成及变化规律带来了新的途径。本文在重点介绍蛋白质免疫印迹技术原理及特点的基础上, 系统地总结了近年来该技术在水产品加工、贮藏、品质鉴别及质量安全方面的应用研究进展, 对目的蛋白质选择的局限性、水产品研究尚未覆盖、蛋白质受环境改变等问题进行了探讨, 就此展望了丰富数据库、多组学方法联合分析等发展前景, 以期为蛋白质免疫印迹技术在水产品品质与质量安全相关研究和应用提供参考与借鉴。

英文摘要:

      As a new method, western blotting technology has been widely used in protein's omics research. This technology can be used for qualitative and half quantitative analysis of the target proteins in the samples to be tested, which brings a new way to explore the composition and variation of protein in aquatic products. On the basis of introducing the principle and characteristics of protein western blotting technology, this paper systematically summarized the recent progresses of western blotting application system at actually in terms of processing, storage, quality identification and quality safety of aquatic products, discussed the limitations of the choice of destination protein, the lack of coverage of aquatic products research, and the environmental changes in protein, prospected the development prospect of riching database, multi-omics method joint analysis and that like, in order to provide references for the research and application of protein western blotting technology in aquatic product quality and quality safety.