气相色谱-三重四极杆串联质谱法同时测定茶叶中16种多环芳烃

Simultaneous determination of 16 kinds of polycyclic aromatic hydrocarbons in tea by gas chromatography-triple quadrupole tandem mass spectrometry

中文关键词:  茶叶, 多环芳烃  分子印迹固相萃取  气相色谱-三重四极杆串联质谱法

英文关键词:tea  polycyclic aromatic hydrocarbons  molecular imprinted polymer solid phase extraction  gas chromatography-triple quadrupole tandem mass spectrometry

中文摘要:

      目的 建立同时测定不同类别茶叶中16种多环芳烃的气相色谱-三重四极杆串联质谱法。方法 茶叶样品粉碎均匀后, 用正己烷超声波萃取, 分子印迹固相萃取柱净化, 氮吹浓缩后, 用气相色谱-三重四极杆串联质谱法检测目标物, 外标法定量。结果 茶叶中16种多环芳烃在5~2500 μg/L范围内线性关系良好, 相关系数(r)在0.9986~0.9999之间, 在绿茶和黑茶样品中添加20、100、500 μg/kg 3个浓度水平的平均加标回收率在80.1%~104.4%之间, 相对标准偏差不大于8.0%, 各目标物的检出限范围为在0.4~4.3 μg/kg之间, 定量限为1.3~14.3 μg/kg。结论 该方法操作简单快捷、灵敏度高、准确度高、选择性良好、线性范围宽, 各目标物分离效果好, 能有效地降低茶叶样品的基质干扰, 能够作为茶叶中的16种多环芳烃含量同时检测的确证方法。

英文摘要:

      Objective To establish a method for the simultaneous determination of 16 kinds of polycyclic aromatic hydrocarbons in various teas by gas chromatography-triple quadrupole tandem mass spectrometry. Methods The tea samples were crushed, extracted under ultrasonication using hexane, cleaned up by molecular imprinted polymer solid phase extraction column, blown and concentrated by nitrogen, analyzed by gas chromatography-triple quadrupole tandem mass spectrometry, and quantified by external standard method. Results The 16 kinds of polycyclic aromatic hydrocarbons showed good linearity in the range of 5-2500 μg/L, and the correlation coefficients (r) was 0.9986?0.9999, the average recoveries from the green tea and dark tea samples at the 3 concentration levels of 20, 100, 500 μg/kg were in the range of 80.1%?104.4%, and the relative standard deviations were no more than 8.0%, the limits of detection were 0.4?4.3 μg/kg, and the limits of quantification were 1.3?14.3 μg/kg. Conclusion This method is simple and fast, has high sensitivity, high accuracy, good selectivity and wide linear range, the separation effect of each target substance is good, and the matrixes interference of tea samples can be effectively reduced, and can be used as a method for the simultaneous determination of 16 kinds of polycyclic aromatic hydrocarbons in tea.