不同生长阶段墨瑞鳕肌肉中氨基酸品质主成分评价

Principal component evaluation of amino acid quality in muscle of Maccullochella peelii at different growth stages

中文关键词:  墨瑞鳕  氨基酸品质  主成分分析

英文关键词:Maccullochella peelii  amino acid quality  principal component analysis

中文摘要:

      目的 研究不同生长阶段墨瑞鳕肌肉中氨基酸含量的差异及其品质评价。方法 采用氨基酸自动分析仪测定墨瑞鳕处于鱼苗、成鱼和亲鱼等3种阶段时肌肉中氨基酸含量, 并通过主成分分析对氨基酸品质进行评价。结果 不同生长阶段墨瑞鳕肌肉及饲料均含有17种氨基酸, 均以谷氨酸含量最高, 胱氨酸含量最低, 而且肌肉与饲料中氨基酸呈强正相关(r2为0.98682~0.99919)。主成分分析显示墨瑞鳕肌肉中17种氨基酸可以降维成2种主成分, 苯丙氨酸和赖氨酸是墨瑞鳕的主要特征氨基酸, 构建的氨基酸品质主成分评价函数式为F=0.756Y1+0.243Y2, 运算结果为幼鱼的得分指最高(4.223), 亲鱼次之(?1.854), 成鱼最低(?2.369)。结论 幼鱼阶段墨瑞鳕肌肉中氨基酸品质最优, 亲鱼次之, 就氨基酸而言, 建议不同生长阶段墨瑞鳕饲料中氨基酸成分的配制以幼鱼及亲鱼饲料作为参考更有效。

英文摘要:

      Objective To study the difference of amino acid content in the muscle of Maccullochella peelii at different growth stages and its quality evaluation of amino acid. Methods The amino acid content in the muscle of Maccullochella peelii at 3 stages of juvenile, adult and parent fish were determined by automatic amino acid analyzer, and the amino acid quality was evaluated by principal component analysis. Results There were 17 kinds of amino acids in the muscle and feed of Maccullochella peelii at different growth stages, and glutamic acid content was the highest, cystine content was the lowest, and there was a strong positive correlation between muscle and feed amino acid (r2=0.98682~0.99919). The principal component analysis showed that the 17 kinds of amino acids in the muscle of Maccullochella peelii could be reduced in dimension into 2 kinds of principal components, and phenylalanine and lysine were the main characteristic amino acids of Maccullochella peelii, the principal component evaluation function of amino acid quality constructed was F=0.756Y1+0.243Y2, and the calculation result showed that juvenile fish had the highest score (4.223), followed by parent fish (?1.854), adult fish had the lowest (?2.369). Conclusion The amino acid quality in the muscle of Maccullochella peelii at juvenile stage is the best, followed by parent fish, in terms of amino acids, it is suggested that the amino acid composition in the feed of Maccullochella peelii at different growth stages should be more effective to refer to the diets of juvenile fish and parent fish.