L-阿拉伯糖大鼠28 d重复剂量经口毒性研究

Study on the L-arabinose 28 d repeated dose oral toxicity in rats

中文关键词:  L-阿拉伯糖  大鼠  亚急性  毒性

英文关键词:L-arabinose  rat  sub-acute study  toxicity

中文摘要:

      目的 研究L-阿拉伯糖对大鼠的亚急性经口毒性。方法 根据GB 15193.22—2014《食品安全国家标准 28天经口毒性试验》, 选取无特定病原体(specific pathogen free, SPF)级健康SD (Sprague-Dawley)大鼠, 依体质量随机分成4组, 将L-阿拉伯糖分别按照0.00、2.50、5.00、10.00 g/kg掺入基础饲料, 连续饲喂28 d, 同时加设对照组和高剂量组2个卫星组, 恢复期为14 d。主试验和恢复期结束时, 称量空腹体质量和主要脏器湿重, 采集血样, 进行血液学、血清生化和组织病理学检测。结果 与对照组相比, L-阿拉伯糖可有效降低雌、雄大鼠的摄食量和体质量, 且正常饮食后可恢复正常; 未见L-阿拉伯糖对大鼠一般状况、眼、食物利用率、尿常规、血液学、血生化、脏器湿重、脏器系数、大体解剖及组织病理学产生有毒理学意义的影响。结论 在本研究条件下, L-阿拉伯糖对雌、雄大鼠28 d经口毒性无可见有害作用剂量(no observed adverse effect level, NOAEL)值分别为13.67和13.92 g/kg(分别相当于15 g/d推荐摄入量的55和56倍)。

英文摘要:

      Objective To evaluate sub-acute oral toxicity of L-arabinose in rats. Methods According to GB 15193.22—2014 National food safety standard-Repeated dose 28-day oral toxicity study, specific pathogen free (SPF) grade healthy Sprague-Dawley (SD) rats were selected and divided randomly based on their bodyweights into 4 groups, L-arabinose were administered via diet at dose levels of 0.00, 2.50, 5.00 and 10.00 g/kg for 28-day, satellite groups of control group and high dose group were added, and the recovery period was 14 d. At the end of the treatment and recovery periods, empty body and the main organs were weighed and blood samples were collected for the hematology, serum biochemistry and histopathology inspection. Results Compare with the control group, L-arabinose could significant decrease the body weights and food intakes of male and female rats, and the indicators could recovered after treatment-free; there were no L-arabinose-related toxicological implications on clinical observations, eyes, food utilization, urine routine, hematology, blood chemistries, organ weights, organ/body weight ratios, gross necropsies as well as histopathology in rats. Conclusion Under the conditions of this study, the no observed adverse effect levels (NOAEL) for L-arabinose on male and female rats are determined as 13.67, 13.92 g/kg respectively (which are 55 and 56 times of 15 g/d for recommended dose in adults) when administered via diet for 28 consecutive days.