高效液相色谱法同时测定护眼保健品中叶黄素和玉米黄质

Simultaneous determination of lutein and zeaxanthin in eye-protecting products by high performance liquid chromatography

中文关键词:  高效液相色谱法  叶黄素  玉米黄质  护眼保健品

英文关键词:high performance liquid chromatography  lutein  zeaxanthin  eye-protecting products

中文摘要:

      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)同时测定护眼保健品中叶黄素和玉米黄质的分析方法。方法 样品用胰酶氯化钠预处理后, 经无水乙醇及石油醚提取, 在55 ℃水浴中用40%氢氧化钾-甲醇溶液皂化30 min, 无水乙醇稀释后, 选用色谱柱YMC Carotenoid S-5 (250 mm× 4.6 mm, 5 μm), 以甲醇:三氯甲烷(4:1, V:V)为流动相, 流速为1.5 mL/min, 柱温为25 ℃, 于445 nm波长处测定样品。结果 叶黄素在0.3906~12.4979 μg/mL范围内线性关系良好, 相关系数r2为0.9999, 平均回收率为92.6%~93.5% (n=9), 检出限为0.0075 μg/g; 玉米黄质在0.1474~4.7184 μg/mL范围内线性关系良好, 相关系数r2为1, 平均回收率在99.8%~108.8% (n=9), 检出限为0.0120 μg/g。结论 该方法快速、稳定, 能够适用于护眼保健品中叶黄素和玉米黄质的同时测定。

英文摘要:

      Objective To establish a method for the simultaneous determination of lutein and zeaxanthin in eye-protecting products by high performance liquid chromatography (HPLC). Methods The samples were pretreated with trypsin sodium chloride, extracted with absolute ethanol and petroleum ether, saponified with 40% potassium hydroxide-methanol solution in 55 ℃ water bath for 30 min, and diluted with absolute ethanol. The chromatographic column YMC Carotenoid S-5 (250 mm×4.6 mm, 5 μm) was selected, methanol:chloroform (4:1, V:V) was used as the mobile phase, the flow rate was 1.5 mL/min, the column temperature was 25 ℃, and the sample was determined at the wavelength of 445 nm. Results The linear range of lutein was 0.3906?12.4979 μg/mL with correlation coefficient (r2) of 0.9999, average recoveries were 92.6%?93.5% (n=9), and the detection limit was 0.0075 μg/g. The linear range of zeaxanthin was 0.1474?4.7184 μg/mL with correlation coefficient (r2) of 1, average recoveries were 99.8%?108.8% (n=9) and the detection limit was 0.0120 μg/g. Conclusion This method is rapid, stable and suitable for the simultaneous determination of lutein and zeaxanthin in eye-protecting products.