高效液相色谱法测定枸杞相关食品中枸杞酸和玉米黄质二棕榈酸酯的含量

Determination of ascorbic acid 2-glucoside and dipalmityl zeaxanthin in Lycium barbarum related foods by high performance liquid chromatography

中文关键词:  高效液相色谱法  枸杞  枸杞提取物  枸杞酸  玉米黄质二棕榈酸酯

英文关键词:high performance liquid chromatography  Lycium barbarum fruit  Lycium barbarum fruit extract  ascorbic acid 2-glucoside  dipalmityl zeaxanthin

中文摘要:

      目的 建立高效液相色谱法分析枸杞原料、提取物和相关配方产品中枸杞酸和玉米黄质二棕榈酸酯的含量。方法 采用Poroshell 120 HILIC柱, 以乙腈-0.1% (V:V)磷酸水(90:10, V:V)为流动相进行分离枸杞酸, 在紫外检测波长235 nm下进行检测, 外标法定量。用Dionex Acclaim C30柱, 以甲醇-甲基叔丁基醚为流动相进行梯度分离玉米黄质二棕榈酸酯, 在紫外检测波长452 nm下进行检测, 外标法定量。结果 枸杞酸的检出限为10 mg/kg, 定量限为30 mg/kg; 在10~200 mg/L的质量浓度范围内线性良好, 相关系数为0.9999; 枸杞原料、提取物和相关配方产品中的加标回收率在84.3%~98.1%之间, 相对标准偏差(relative standard deviations, RSDs)为1.3%~4.7%; 精密度RSDs为0.65%~1.20%; 重复性RSDs为1.3%~3.1%。玉米黄质二棕榈酸酯的检出限为10 mg/kg, 定量限为30 mg/kg; 在0.5~10.0 mg/L的浓度范围内线性良好, 相关系数为0.999; 枸杞原料、提取物和相关配方产品中的加标回收率为83.8%~98.6%, RSDs为1.5%~4.5%; 精密度RSDs为1.2%~2.1%; 重复性RSD为2.0%~4.5%。结论 本方法操作简便, 快速, 分离度和准确度高, 可用于枸杞原料、提取物和相关配方产品的质量控制。

英文摘要:

      Objective To establish a method for the determination of ascorbic acid 2-glucoside (AA-2βG) and zeaxanthin dipalmitate content in raw materials, extracts and related formula products of Lycium barbarum by high performance liquid chromatography. Methods AA-2βG was separated on a poroshell 120 HILIC column, eluted by acetonitrile-0.1% (V:V) phosphoric acid water solution (90:10, V:V), identified by ultraviolet (UV) detector at 235 nm and quantified by external standard method. Dipalmityl zeaxanthin was separated on a Dionex Acclaim C30 column, eluted by methanol-methyl tert-butyl ether, identified by UV detector at 452 nm, and quantified by external standard method. Results For AA-2βG, the limits of detection (LODs) and quantification (LOQs) were 10 and 30 mg/kg respectively; the linearity was good in the mass concentration range of 10?200 mg/L, and the correlation coefficient was 0.9999; the recoveries of AA-2βG in Lycium barbarum fruit raw material, extract and the related formulated food varied from 84.3% to 98.1% and the relative standard deviations (RSDs) varied from 1.3% to 4.7%; the precision RSDs varied from 0.65% to 1.20%, and the repeatability RSDs varied from 1.3% to 3.1%. For dipalmityl zeaxanthin, the LOD and LOQ were 10 and 30 mg/kg respectively; the linearity was good in the concentration range of 0.5?10.0 mg/L, and the correlation coefficient was 0.999; the recoveries of dipalmityl zeaxanthin in Lycium barbarum fruit raw material, extract and the related formulated food varied from 83.8% to 98.6% and the RSDs varied from 1.5% to 4.5%. The precision RSDs varied from 1.2% to 2.1%, and the repeatability RSDs varied from 2.0% to 4.5%. Conclusion The method is simple, rapid, and has high degree of separation and accuracy, which can be used for the quality control of Lycium barbarum fruit raw material, extract and the related formulated food.