胶体金免疫层析法同时检测果蔬中克百威、水胺硫磷、百菌清及虫酰肼的应用研究

Application research on the simultaneous determination of carbofuran, hydrocarbophos, chlorothalonil and tebufenozide in fruits and vegetables by colloidal gold immunochromatography

中文关键词:  胶体金免疫层析法  克百威  水胺硫磷  百菌清  虫酰肼  果蔬

英文关键词:colloidal gold immunochromatography  carbofuran  hydrocarbophos  chlorothalonil  tebufenozide  fruits and vegetables

中文摘要:

      目的 研究胶体金免疫层析法同时检测果蔬中克百威、水胺硫磷、百菌清及虫酰肼残留量的适用性。方法 考察胶体金免疫层析法同时检测克百威、水胺硫磷、百菌清及虫酰肼在不同基质中的灵敏度, 并将该方法与参比方法进行比较, 验证其假阴性率和假阳性率。结果 克百威检出限为0.02 mg/kg, 水胺硫磷为 0.05 mg/kg、百菌清为3 mg/kg、虫酰肼为0.2 mg/kg, 基本满足国家标准对果蔬中这些农药的限量要求。对韭菜、大白菜进行加标实验, 均未出现假阳性及假阴性, 与参比方法无显著差异; 除丙硫克百威、丁硫克百威与克百威有交叉反应外, 水胺硫磷、百菌清及虫酰肼与非对应农药均无交叉反应。结论 该方法操作快速、准确、灵敏、简便, 适用于果蔬中克百威、水胺硫磷、百菌清及虫酰肼的同时检测。

英文摘要:

      Objective To study the reliability for the simultaneous determination of carbofuran, hydrocarbophos, chlorothalonil and tebufenozide residues in fruits and vegetables by colloidal gold immunochromatography. Methods The experiment verified the sensitivity of colloidal gold immunochromatography for detecting carbofuran, hydrocarbophos, chlorothalonil and tebufenozide in different substrates, this method was compared with the reference standard method to verify the false negative and false positive rate of the test results. Results The limit of detection of carbofuran was 0.02 mg/kg, hydrocarbophos was 0.05 mg/kg, chlorothalonil was 3 mg/kg, and tebufenozide was 0.2 mg/kg, basically met the national standard limit requirements for these pesticides in fruits and vegetables. The standard addition test for leeks and Chinese cabbage showed no false positives and false negatives, which was not significantly different from the reference method; except for the cross-reaction of benfuracarb, carbosulfan and carbofuran, hydrocarbophos, chlorothalonil and tebufenozide had no cross-reaction with non-corresponding pesticides. Conclusion This method is fast, accurate, sensitive and simple, which is suitable for the simultaneous determination of carbofuran, hydrocarbophos, chlorothalonil and tebufenozide in fruits and vegetables.