含胶原蛋白肽饮品对衰老小鼠皮肤改善作用和抗氧化作用研究

Study of skin improvement and antioxidant activity in aged mice with collagen peptide drinks

中文关键词:  胶原蛋白肽  衰老小鼠  抗氧化  短链脂肪酸

英文关键词:collagen peptide  aged mouse  antioxidant  short chain fatty acid

中文摘要:

      目的 研究3款含胶原蛋白肽饮品对衰老小鼠的皮肤改善作用及抗氧化作用。方法 采取D-半乳糖诱导的衰老小鼠模型, 正常对照组每天腹腔注射生理盐水, 模型组和产品组每天对小鼠腹腔注射D-半乳糖120 mg/kg。各组小鼠每天按0.1 mL/10 g体积灌胃, 模型组及对照组灌胃生理盐水, 产品组灌胃相应体积样品, 连续8周, 各实验组剂量16.7 mL/(kg·d)。分别检测皮肤羟脯氨酸和透明质酸含量, 血清、肝脏、心脏中超氧化物歧化酶(superoxide dismutase, SOD)活性、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)活性和丙二醛(malondialdehyde, MDA)含量, 小肠和结肠的pH、短链脂肪酸含量。结果 相对于模型组, 3款胶原蛋白肽饮品均能提高衰老模型小鼠皮肤透明质酸和羟脯氨酸的含量、提升衰老小鼠的抗氧化能力(P<0.05), 表现为提高血清、肝脏、心脏的SOD和GSH-Px活性, 以及降低MDA浓度, 且3款饮品中以UKDNA胶原蛋白肽饮品的效果最好。对小肠、结肠内容物pH和短链脂肪酸(short chain fatty acids, SCFAs)含量检测显示, 3款饮品能提高结肠环境pH并对异丁酸、正戊酸以及总短链脂肪酸有显著影响(P<0.05)。结论 含胶原蛋白肽饮品可能通过提高机体抗氧化能力来改善衰老皮肤中透明质酸和羟脯氨酸含量。

英文摘要:

      Objective To study the skin improvement effects and antioxidant activity by 3 kinds of collagen peptide-contained drinks on aged mice. Methods The aged mouse model induced by D-galactose was adopted, the normal control group was intraperitoneally injected with normal saline every day, and the model group and product group were intraperitoneally injected with D-galactose 120 mg/kg every day. The mice in each group were gavaged at the volume of 0.1 mL/10 g every day, the model group and control group were gavaged with salt water, the product group was gavaged with the corresponding volume of samples for 8 consecutive weeks, and the dose of each experimental group was 16.7 mL/(kg·d). The content of hydroxyproline and hyaluronic acid in skin, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, malondialdehyde (MDA) content in serum, liver and heart, pH and short chain fatty acid content in small intestine and colon were measured. Results Compared with model group, the 3 kinds of collagen peptide-contained drinks could increase the content of hyaluronic acid and hydroxyproline in the skin of aging model mice, and enhance the antioxidant capacity of aged mice (P<0.05), which were shown as the increasing activities of SOD and GSH-Px in serum, liver and heart and the reduction of MDA, and UKDNA collagen peptide-contained drink had the best effects among the 3 kinds of samples. Additionally, the results showed that the 3 kinds of samples could improve the pH and significant affected the concentrations of isobutyric acid and valerate acid and total SCFAs in colon (P<0.05). Conclusion Collagen peptide-contained drinks may improve the content of hyaluronic acid and hydroxyproline in skin in aged mice through increasing antioxidant capacity in body.