QuEChERS-超高效液相色谱-串联质谱法测定2种茄果类有机蔬菜中三唑酮及其代谢物

Determination of triadimefon and its metabolites in 2 kinds of solanaceous organic vegetables by QuEChERS-ulra performance liquid chromatography- tandem mass spectrometry

中文关键词:  QuEChERS  超高液相色谱-串联质谱法  三唑酮  三唑醇  茄果类有机蔬菜

英文关键词:QuEChERS  ultra performance liquid chromatography-tandem mass spectrometry  triadimefon  triadimenol  solanaceous organic vegetables

中文摘要:

      目的 建立QuEChERS结合超高效液相色谱-串联质谱法(ultra performance liquid chromatography -tandem mass spectrometry, UPLC-MS/MS)测定2种茄果类蔬菜中三唑酮及其代谢产物三唑醇对映异构体的分析方法。方法 采用乙腈作为提取溶剂, N-丙基乙二胺(primary secondary amine, PSA)混合石墨化碳黑净化, 样品经CHIRAL?OD-RH手性柱分离, 采用UPLC-MS/MS测定, 基质外标法定量。结果 三唑酮和三唑醇在0.02~1.00 mg/L范围内线性良好, 相关系数均在0.99以上, 定量限(limits of quantitation, LOQ)为 0.02 mg/L。有机茄子、有机青椒中3个添加水平的平均回收率为71.0%~97.8%, 相对标准偏差(relative standard deviation, RSD) (n=6)在1.2%~9.9%之间。结论 该方法操作简便, 准确度高、成本低, 可以用于测定茄果类有机蔬菜中三唑酮及代谢物三唑醇残留。

英文摘要:

      Objective To establish a method for the determination of triadimefon and its metabolite triadimenol enantiomers in 2 kinds of solanaceous vegetables by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods Acetonitrile was used as the extraction solvent, primary secondary amine (PSA) was mixed with graphitized carbon black and the samples were purified by CHIRAL?OD-RH chiral column separation, determined by UPLC-MS/MS and quantified by matrix external standard method. Results The linear range for triadimefon and triadimenol were 0.02?1.00 mg/L, the correlation coefficients were all above 0.99, and limits of quantitation (LOD) was 0.02 mg/L. The average recoveries rate of the 3 addition levels in organic eggplant and organic green pepper were 71.0%?97.8%, and the relative standard deviations (RSDs) (n=6) were 1.2%?9.9%. Conclusion The method is simple to operate, has high accuracy and low cost, and can be used to determine triadimefon and metabolite residues in solanaceous organic vegetables.