高效液相色谱法测定保健食品-复合营养素补充剂中维生素B1含量

Determination of vitamin B1 in functional food-complex nutrients supplementation by high performance liquid chromatography

中文关键词:  维生素B1  多种维生素矿物质片  复合营养素补充剂  保健食品  高效液相色谱法

英文关键词:vitamin B1  compound vitamins and minerals tablets  complex nutrients supplementation  functional food  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)测定保健食品-复合营养素补充剂中维生素B1(vitamin B1, VB1)含量的分析方法。方法 选取基质复杂的多种维生素矿物质片作为代表样品, 通过创新性地设计酸性饱和乙二胺四乙酸二钠盐(ethylenediamine tetraacetic acid disodium salt, EDTA)提取溶液结合超声提取前处理方法, 充分络合各类矿物质元素并有效消除其他基质干扰, 针对样品中的维生素B1进行高效率提取。提取液经0.45 μm滤膜过滤后, HPLC分离检测。结果 此改进后的分析方法能够针对多种维生素矿物质片中维生素B1含量进行准确定量检测。维生素B1在1.0237~204.7380 μg/mL浓度范围内线性关系良好(r2=0.9999), 方法的平均回收率达98.59%, 相对标准偏差(relative standard deviations, RSDs)为2.08% (n=9), 方法定量限(limit of quantification, LOQ)为0.05 μg/mL。结论 本方法简单、高效、抗基质干扰能力强、重现性好, 适用于多种维生素矿物质片中的维生素B1的含量测定, 同时也为复杂基质的保健食品-复合营养素补充剂中维生素B1的含量检测提供新的途径。

英文摘要:

      Objective To establish a method for the determination of vitamin B1 in functional food-complex nutrients supplementation by high performance liquid chromatography (HPLC). Methods Multi vitamin mineral tablets with complex matrix were selected as representative samples. By innovatively designing the extraction solution of acid saturated ethylenediamine tetraacetic acid disodium salt (EDTA) and combining the pretreatment method of ultrasonic extraction, various mineral elements were fully complexed and other matrix interference was effectively eliminated, and vitamin B1 in the sample was extracted with high efficiency. The extract was filtered by 0.45 μm filter membrane and separated and detected by HPLC. Results Using this improved method could accurately and quantitatively detect vitamin B1 in compound vitamins and minerals tablets. In the range of 1.0237?204.7380 μg/mL, the linear relationship of vitamins was good (r2=0.9999). The average recoveries were 98.59%. The relative standard deviations (RSDs) were 2.08% (n=9). This limit of quantification of method was 0.05 μg/mL. Conclusion This method is simple, efficient, has strong anti matrix interference ability and good reproducibility, and can be used for determination of vitamin B1 in compound vitamins and minerals tablets. It also provides a new approach for determination of vitamin B1 in functional food-complex nutrients supplementation with complex matrix.