QuEChERS结合高效液相色谱法测定食品接触用耐高温纸制品中14种有机抗菌剂

Determination of 14 kinds of organic antimicrobials in high temperature resistant paper products for food contact by QuEChERS combined with high performance liquid chromatography

中文关键词:  QuEChERS  高效液相色谱法  食品接触用纸  耐高温纸制品  有机抗菌剂

英文关键词:QuEChERS  high performance liquid chromatography  food contact paper  high temperature resistant paper products  organic antimicrobials

中文摘要:

      目的 建立QuEChERS结合高效液相色谱法(high performance liquid chromatography, HPLC)测定食品接触用耐高温纸制品中14种有机抗菌剂的方法。方法 样品用乙腈-水溶液(1:1, V:V)超声提取, QuEChERS方法净化, C18色谱柱(250 mm×4.6 mm, 5 μm)分离, 以乙腈、甲醇、0.1%乙酸溶液为流动相进行梯度洗脱, 二极管阵列检测器于191~400 nm检测, 外标法定量。结果 14种有机抗菌剂在0.05~20.00 mg/L范围内呈良好线性关系, r≥0.996; 方法检出限均为0.05 mg/kg, 定量限均为0.15 mg/kg; 平均加标回收率为73.8%~96.3%, 相对标准偏差为2.8%~5.5%。结论 该方法操作简单、快速、准确可靠, 适用于食品接触用耐高温纸制品中14种有机抗菌剂的检测。

英文摘要:

      Objective To establish a method for the determination of 14 kinds of organic antimicrobials in high temperature resistant paper products for food contact by QuEChERS combined with high performance liquid chromatography (HPLC). Methods The samples were extracted ultrasonically with acetonitrile-water solution (1:1, V:V), purified by QuEChERS, separated by C18 (250 mm×4.6 mm, 5 μm) column combined with acetonitrile, methanol and 0.1% acetic acid solution as mobile phases for gradient elution, detected by diode array detector (DAD) at 191?400 nm, and quantified by external standard method. Results The calibration curves of the 14 kinds of organic antimicrobials showed good linear relationships in the range of 0.05?20.00 mg/L with the correlation coefficients great than or equal to 0.996. The limits of detection (LODs) and the limits of quantification (LOQs) of the 14 kinds of organic antimicrobials were 0.05 mg/kg and 0.15 mg/kg, respectively. The average recoveries were in the range of 73.8%?96.3% with relative standard deviations (RSDs) in the range of 2.8%?5.5%. Conclusion The method is simple, fast, accurate and reliable, and suitable for the detection of the 14 kinds of organic antimicrobials in high temperature resistant paper products for food contact.