巫山茶多酚提取物体外抗氧化活性研究

Study on the in vitro antioxidant activity of the Wushan tea polyphenol extract

中文关键词:  巫山茶  多酚提取物  萃取相  大孔树脂  体外抗氧化活性

英文关键词:Wushan tea  polyphenol extract  extract phase  macroporous resin  in vitro antioxidant activity

中文摘要:

      目的 初步评价巫山茶多酚提取物的体外抗氧化活性。方法 采用70%乙醇提取巫山茶得到巫山茶粗提物, 再依次使用不同极性溶剂萃取巫山茶粗提物, 得到石油醚相、乙酸乙酯相、正丁醇相和水相4个萃取相, 分别测定各萃取相的总酚含量和体外抗氧化活性, 选取总酚含量最高且体外抗氧化活性最强的萃取相通过HP-20大孔树脂进行富集, 测定富集后的巫山茶多酚提取物总酚含量和体外抗氧化活性。结果 乙酸乙酯相的总酚含量最高(378.1 mg/g); 体外抗氧化活性最强, DPPH·、ABTS+·、·OH的IC50值分别为0.63、0.46、2.35 mg/mL; 将乙酸乙酯相通过HP-20大孔树脂富集后得到的巫山茶多酚提取物总酚含量为637.5 mg/g, 总酚含量显著提高(P<0.05), DPPH·、ABTS+·、·OH的IC50值分别为0.26、0.16、1.05 mg/mL, 体外抗氧化活性显著增强(P<0.05)。相关性分析结果显示, 巫山茶粗提物及各萃取相、富集后的总酚含量与其体外抗氧化活性呈正相关。结论 巫山茶多酚提取物具有较好的体外抗氧化活性。

英文摘要:

      Objective To preliminarily evaluate the in vitro antioxidant activity of Wushan tea polyphenol extract. Methods The crude extract of Wushan tea was extracted with 70% ethanol, then the crude extract of Wushan tea was extracted with different polar solvents successively, and 4 kinds of extraction phases were obtained: Petroleum ether phase, ethyl acetate phase, n-butanol phase and water phase. The total phenol content and the in vitro antioxidant activity of each extract phase were determined, the extract phase with the highest total phenol content and the strongest in vitro antioxidant activity was enriched by HP-20 macroporous resin, and the total phenol content and the in vitro antioxidant activity of the enriched Wushan tea polyphenol extract were determined. Results The total phenol content of ethyl acetate phase was the highest (378.1 mg/g) and the ethyl acetate phase had the strongest in vitro antioxidant activity, the IC50 values of DPPH·, ABTS+· and ·OH were 0.63, 0.46 and 2.35 mg/mL respectively. After enriched by HP-20 macroporous resin, the total phenol content in the ethyl acetate phase was 637.5 mg/g, which was significantly increased (P<0.05). The IC50 values of DPPH·, ABTS+· and ·OH were 0.26, 0.16 and 1.05 mg/mL respectively, showed that the in vitro antioxidant activity was significantly enhanced (P<0.05). The correlation analysis result showed that the total phenol content of the crude extract of Wushan tea and its extract phases, and the enriched Wushan tea polyphenol extract were positively correlated with their in vitro antioxidant activity. Conclusion The Wushan tea polyphenol extract have good in vitro antioxidant activity.