不同品种白茶水提物对果蝇热耐受能力的影响

Effects of water extract from different kinds of white tea on heat tolerance of Drosophila melanogaster

中文关键词:  白茶水提物  黑腹果蝇  热暴露  热锻炼  热耐受能力

英文关键词:white tea water extract  Drosophila melanogaster  heat exposure  heat hardening  heat tolerance

中文摘要:

      目的 探究不同品种白茶水提物对高温胁迫下果蝇存活能力的影响, 筛选对热应激有防护作用的白茶品种。方法 选取黑腹果蝇为模式生物, 在基础培养基中分别添加不同茶水比[3:100、5:100、7:100、9:100、11:100 (g/mL)]浸提的白毫银针、白牡丹、贡眉及寿眉水提物, 观察各培养基饲喂的果蝇在36 ℃热暴露下的存活情况。选取提高果蝇热耐受能力最佳的白茶品种进行热锻炼实验。结果 适当茶水比浸提的4种白茶水提物不同程度地延长了雌性果蝇的半数致死时间(lethal time of 50%, LT50)、平均存活时间(mean survival time, T)及最高存活时间(maximum survival time, Tmax), 提高了雌性果蝇热耐受能力, 但不存在剂量-效应关系。白毫银针水提物改善雌性果蝇热耐受能力的作用最佳, 4种白茶水提物改善雌性果蝇热耐受能力对应的最佳茶水比均为9:100 (g/mL)。热锻炼可提高雌性果蝇的热适应能力, 9:100 g/mL茶水比浸提的白毫银针水提物极显著地改善了热锻炼后雌性果蝇的热耐受能力(P<0.01)。结论 本研究结果可为白茶摄入改善机体对高温的耐受性提供理论依据。

英文摘要:

      Objective To investigate the effect of water extract from different kinds of white tea on the survival of Drosophila melanogaster under high temperature stress, and screen white tea variety with protective effect on heat stress. Methods Selecting Drosophila melanogaster as the heat-exposed organisms, water extract of Bai Hao silver needle, Baimudan, Gongmei and Shoumei were added to the basal medium with different tea water ratios [3:100, 5:100, 7:100, 9:100, 11:100 (g/mL)], respectively, and the survival of female Drosophila melanogaster was observed at 36 ℃. White tea variety with the best heat tolerance for Drosophila melanogaster was selected for the heat hardening test. Results The lethal time of 50% (LT50), average survival time (T) and the maximum survival time (Tmax) of female Drosophila melanogaster to varying degrees were prolonged by the 4 white tea extracts extracted with appropriate tea water ratio. The thermal tolerance of female Drosophila melanogaster was improved, but there was no dose-response relationship. The water extract of Bai Hao silver needle had the best effect on improving the thermal tolerance of female Drosophila melanogaster. The optimal tea/water ratio of 4 white tea extracts to improve the thermal tolerance of female Drosophila melanogaster was 9:100 (g/mL). Heat hardening can improve the thermal adaptability of female Drosophila melanogaster, and the heat tolerance of female Drosophila melanogaster after heat tolerance was significantly improved by 9:100 (g/mL) tea water compared with the water extract of Bai Hao silver needle (P<0.01). Conclusion The results of this study can provide a theoretical basis for improving the tolerance of body to high temperature by white tea intake.