磁珠法提取植物蛋白饮料DNA

Extraction of plant protein beverage DNA by magnetic beads

中文关键词:  磁珠法  植物蛋白饮料  DNA  检测

英文关键词:method of magnetic beads  plant protein beverage  DNA  detection

中文摘要:

      目的 建立一种快速、高效的植物蛋白饮料基因组DNA提取方法并应用于植物蛋白饮料中植物源性成分的检测。方法 以豆浆、豆奶、芝麻糊、花生牛奶、榛子乳5种植物蛋白饮料为研究对象, 建立利用硅羟基磁珠提取植物蛋白饮料DNA的方法, 通过分析所提取DNA的浓度和纯度, 实时荧光PCR扩增效率, 并与十六烷基三甲基溴化铵法(cetyltrimethylammonium ammonium bromide, CTAB)比较, 综合评价磁珠法提取植物蛋白饮料的效果。结果 大部分样品磁珠法提取的DNA浓度比CTAB法高, ODA260/A280均大于1.70。 结论 磁珠法提取的DNA纯度较高, 杂质少, 能满足植物蛋白饮料源性成分的分析要求, 尤其适用于大豆类蛋白饮料的DNA提取, 为快速、高效获取质量好的植物蛋白饮料基因组DNA提供参考。

英文摘要:

      Objective To establish a rapid, efficient plant protein beverage genomic DNA extraction method and apply to the detection of plant-derived components in plant protein beverage. Methods Five kinds of plant protein drinks, including soybean milk, soy milk products, sesame paste, peanut milk and hazelnut milk, were used as research objects to establish a method to extract DNA from plant protein drinks by using silicon hydroxyl magnetic beads. Comparing with CTAB method, the effect of magnetic beads method on the extraction of plant protein beverage were comprehensively evaluated by analyzing the concentration and purity of the extracted DNA and the amplification efficiency of real-time fluorescence PCR. Results The concentration of DNA extracted by magnetic beads method was higher than that by CTAB method in most samples. The ODA260/A280 ratio was more than 1.70. Conclusion The DNA extracted by magnetic beads method has higher purity and less impurities, which can achieve the rapid and efficient extraction of genomic DNA from various plant-derived components of plant protein drinks. It is especially suitable for the DNA extraction of soybean protein drinks, and provides a reference for the rapid and efficient acquisition of genomic DNA of good quality plant protein drinks.