偏重亚硫酸钠对微生物法测定维生素B12含量的影响

Influence of sodium metabisulfite on the determination of vitamin B12 by microbiological method

中文关键词:  维生素B12  微生物法  偏重亚硫酸钠  莱士曼氏乳杆菌

英文关键词:vitamin B12  microbiological method  sodium metabisulfite  Lactobacillus leichmannii

中文摘要:

      目的 探究偏重亚硫酸钠对微生物法测定婴幼儿食品和乳品中维生素B12(vitamin B12, VB12)含量的影响。方法 通过在标准工作液和样品待测液中添加质量浓度梯度为0、0.0025、0.005、0.02、0.08和0.16 mg/mL偏重亚硫酸钠, 测定不同处理组实验样品中VB12含量值并对质控样品进行质控结果分析, 研究偏重亚硫酸钠对莱士曼氏乳杆菌生长的促进作用及其对原生VB12提取的影响。结果 在标准溶液中添加质量浓度为0~ 0.16 mg/mL的偏重亚硫酸钠都可以促进莱士曼氏乳杆菌的生长。在0~0.05 mg/mL的范围内时, 偏重亚硫酸钠能促进样品中原生VB12的提取, 且随偏重亚硫酸钠质量浓度增加, 原生VB12的提取会成比例增加。结论 偏重亚硫酸钠在0~0.16 mg/mL浓度范围内可以促进莱士曼氏乳杆菌的生长, 提高食品原生VB12的提取率, 进而增加检测结果的稳定性和准确度。

英文摘要:

      Objective To explore the effect of sodium metabisulfite on the determination of vitamin B12 (VB12) in infant food and dairy products by microbiological method. Methods By adding mass concentration gradients of 0, 0.0025, 0.005, 0.02, 0.08 and 0.16 mg/mL sodium metabisulfite to the standard working solution and the sample solution, the content of VB12 in the experimental samples of different treatment groups was detected and the results of quality control samples were analyzed, the promotion node of sodium metabisulfite on Lactobacillus leichmannii and its effect on the extraction of the original form of VB12 were explored. Results Adding sodium metabisulfite with a mass concentration of 0?0.16 mg/mL to the standard solution could promote the growth of Lactobacillus leichmannii. Sodium metabisulfite could promote the extraction of native VB12 in the samples in the range of 0?0.05 mg/mL, and as the concentration of sodium metabisulfite increases, the extraction of native VB12 would be proportionally increased. Conclusion In the concentration range of 0?0.16 mg/mL, sodium metabisulfite can promote the growth of Lactobacillus leichmannii, increase the extraction rate of native VB12, and thereby increase the stability and accuracy of the test results.