基于核酸适配体的生物传感技术检测食源性致病菌研究进展

Research progress on the detection of foodborne pathogens by biosensor based on aptamers

中文关键词:  食源性致病菌  核酸适配体  生物传感器

英文关键词:foodborne pathogens  aptamer  biosensor

中文摘要:

      食品供应的全球化导致食源性疾病传播快、分布广, 严重威胁人类健康。病原检测需要特异性强和灵敏度高的方法。核酸适配体是可以识别并与多种类型靶标分子的单链DNA或RNA结合。基于核酸适配体的生物传感器特异性好、灵敏度高、易储存, 为食源性致病菌快速检测提供了新的方法。本文介绍了核酸适配体的特点和筛选技术, 综述了基于核酸适配体的电化学、比色、荧光、表面增强拉曼散射和质量生物传感器技术的基本原理及其在食源性致病菌检测中的应用, 以期为开发高效、精准检测食源致病菌技术提供参考。

英文摘要:

      The globalization of food supply may lead to the rapid spread and wide distribution of foodborne diseases, which seriously endanger human healthy. It is required for the technology of pathogen detection with highly specific and sensitive. Aptamers are single stranded DNA or RNA molecules which can recognize and bind to target molecules. Aptamers based biosensors are more specific and sensitive, easy to store, and provide new methods for rapid detection of foodborne pathogens. This paper introduced the characteristics and screening technology of aptamers, and summarized the basic principles of aptamers based electrochemical, colorimetric, fluorescence, surface enhanced Raman scattering and quality biosensor technology and its application in the detection of foodborne pathogen, in order to provide reference for the development of fast, efficient, and accurate detection technology of foodborne pathogens.