QuEChERS结合高效液相色谱-串联质谱法测定大白菜中10种喹诺酮类药物残留

Determination of 10 kinds of quinolones residues in cabbage by QuEChERS coupled with high performance liquid chromatography-tandem mass spectrometry

中文关键词:  大白菜  高效液相色谱-串联质谱法  喹诺酮类  药物残留

英文关键词:cabbage  high performance liquid chromatography-tandem mass spectrometry  quinolones  drug residues

中文摘要:

      目的 建立QuEChERS结合高效液相色谱-串联质谱法测定大白菜中10种喹诺酮类药物残留的分析方法。方法 大白菜样品经提取, 采用QuEChERS净化方法, 以诺氟沙星-D5为内标, 用高效液相色谱-串联质谱法测定, 采用正离子电喷雾, 多反应监测模式, 内标法定量分析10种喹诺酮类药物残留的含量。结果 10种喹诺酮类在5~100 ng/mL的质量浓度范围内线性良好, 检出限为0.1~0.3 μg/kg, 定量限为0.3~1.0 μg/kg, 平均回收率为76.0%~107.0%, 相对标准偏差为1.5%~8.1%。结论 该方法准确、可靠, 适用于大白菜中喹诺酮类药物残留的检测。

英文摘要:

      Objective To establish a method for the determination of 10 kinds of quinolones residues in cabbage by QuEChERS coupled with high performance liquid chromatography-tandem mass spectrometry. Methods The cabbage samples were extracted, using the QuEChERS purification method and norfloxacin D5 as internal standard, and quantitatively determined by high performance liquid chromatography-tandem mass spectrometry. The content of 10 kinds of quinolones residues were quantitatively analyzed by positive ion electrospray and multi-reaction monitoring mode. Results The 10 kinds of quinolones showed good linearity in the concentration range of 5? 100 ng/mL, the limits of detection were 0.1?0.3 μg/kg, the limits of quantitation were 0.3?1.0 μg/kg, the average recoveries were 76.0%?107.0%, and the relative standard deviations were 1.5%?8.1%. Conclusion This method is accurate, reliable and suitable for the determination of quinolones residues in cabbage.