响应面法优化顶空固相微萃取条件结合气相色谱-质谱法测定四川红茶挥发性成分

Determination of volatile components in Sichuan black tea by response surface optimized headspace solid phase microextraction combined with gas chromatography-mass spectrometry

中文关键词:  响应面法  顶空固相微萃取  气相色谱-质谱法  四川红茶  挥发性成分

英文关键词:response surface methodology  headspace solid phase micro extraction  gas chromatography-mass spectrometry  Sichuan black tea  volatile components

中文摘要:

      目的 优化顶空固相微萃取技术(headspace solid phase microextraction, HS-SPME)对四川红茶挥发性成分的萃取条件。方法 通过单因素实验, 考查萃取时间、萃取温度和样品质量对萃取效果的影响, 并通过Box-Benhnken中心组合实验及响应面分析法对萃取条件进行优化。结果 最佳萃取条件为萃取时间60 min、萃取温度65 ℃、样品质量1.00 g, 验证实验结果与预测值的相对误差为2.10%。在此条件下, 在四川红茶中共检出醇类、醛类、酮类等9类共计56种挥发性成分, β-紫罗酮、芳樟醇、橙花醇、苯乙醛、苯乙醇等9种高相对香气活度值(relative odor activity value, ROAV)的成分可能是四川红茶的特征挥发性成分。结论 优化所得四川红茶挥发性成分的HS-SPME最佳萃取条件, 结合气相色谱-质谱法(gas chromatography-mass spectrometry, GC-MS)进行分析, 方法可靠, 具有较好应用价值。

英文摘要:

      Objective To optimize the extraction conditions of volatile components in Sichuan black tea by headspace solid-phase micro extraction (HS-SPME). Methods The influences of extraction time, temperature and sample quality on extraction effect were studied by single factor experiment, and the extraction conditions were optimized by Box-Benhnken central composite experiment and response surface analysis. Results The optimum extraction conditions were as follows: Extraction time 60 min, extraction temperature 65 ℃, sample mass 1.00 g, and the relative error between the verification test results and the predicted values was 2.10%. Under this conditions, a total of 56 kinds of volatile components were detected in Sichuan black tea, including 9 kinds of alcohols, aldehydes, ketones, etc, and 9 kinds of highly relative odor activity value (ROAV) components such as β-ionone, linalool, nerol, phenylacetaldehyde and phenylethanol, etc, may be the characteristic volatile components of Sichuan black tea. Conclusion The method for the determination of volatile components in Sichuan black tea by optimized HS-SPME extraction conditions combined with GC-MS is reliable and valuable.