蒜酶催化大鼠血浆中同型半胱氨酸作用研究

Catalytic effect of alliinase on homocysteine in rat plasma

中文关键词:  血浆  同型半胱氨酸  蒜酶  分子对接  高效液相色谱法

英文关键词:plasma  homocysteine  alliinase  molecular docking  high performance liquid chromatography

中文摘要:

      目的 研究蒜酶对血浆中同型半胱氨酸的催化裂解作用。方法 采用分子对接的方法对蒜酶与蒜氨酸、同型半胱氨酸、半胱氨酸的结合能打分, 初步探索蒜酶对同型半胱氨酸的催化裂解作用; 通过高效液相色谱法(high performance liquid chromatography, HPLC)检测血浆中的同型半胱氨酸, 研究蒜酶对血浆中的同型半胱氨酸催化裂解作用。结果 蒜氨酸、同型半胱氨酸、半胱氨酸与蒜酶分子对接, 获得其亲和力分别为-5.8、-4.7、-4.4 kcal/mol, 它们与蒜酶具有一定的结合活性, 同型半胱氨酸与蒜酶的反应作用小于蒜氨酸, 而大于半胱氨酸。同型半胱氨酸在10~128 μmol/L浓度范围内线性良好(r=0.9991), 回收率为93.39%~104.55%。蒜酶催化裂解血浆中的同型半胱氨酸转化率达49.59%。结论 蒜酶对血浆中的同型半胱氨酸具有一定的催化裂解作用, 为蒜酶用于心血管系统疾病的预防和治疗提供了研究基础。

英文摘要:

      Objective To study the catalytic cleavage of alliinase on homocysteine in plasma. Methods The molecular docking method was used to score the binding energy of alliinase with alliin, homocysteine and cysteine, and the catalytic cleavage of alliinase on homocysteine was preliminarily explored. Plasma was detected to study the catalytic cleavage of homocysteine in plasma by alliinase by high performance liquid chromatography. Results Alliin, cysteine, and homocysteine were docked with alliinase molecules, and their affinities were -5.8, -4.7, and - 4.4 kcal/mol, respectively, indicating that they had a strong affinity with alliinase. Certain binding activity, but the reaction between homocysteine and alliinase was less than alliin and greater than cysteine. Homocysteine had a good linearity in the concentration ranges of 10?128 μmol/L (r=0.9991), and the recoveries rate were 93.39%?104.55%. Alliinase catalyzed the cleavage of homocysteine in plasma with a conversion rate of 49.59%. Conclusion Alliinase can catalyze the cleavage of homocysteine in plasma, which has certain reference significance for the prevention and treatment of cardiovascular diseases by alliinase.