QuEChERS-超高效液相色谱-串联质谱法测定8种农产品中的丙硫唑残留

Determination of albendazole residues in 8 kinds of agriculture products by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  QuEChERS  超高效液相色谱-串联质谱法  丙硫唑  残留

英文关键词:QuEChERS  ultra performance liquid chromatography-tandem mass spectrometry  albendazole  residue

中文摘要:

      目的 建立超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)快速测定8种农产品中丙硫唑残留的方法。方法 以经典QuEChERS技术为基础, 优化前处理方法的提取剂和净化剂, 1%乙酸-乙腈(V:V)提取, C18净化, 外标法定量。结果 在0.00025~ 0.50 mg/L的质量浓度范围内, 对应质量浓度与峰面积之间具有良好的相关性, 相关系数均大于0.99; 在0.001、0.01和0.1 mg/kg 3个添加水平下的平均回收率在70%~110%之间, 相对标准偏差为0.83%~10.65%, 定量限(limit of quantitation, LOQ)和检出限(limit of detection, LOD)均为0.001 mg/kg。结论 该方法具有快速、简便、成本低廉和准确度高等优点, 适用于大米、烟草、辣椒、西瓜、白菜、小麦、葡萄和香蕉8种农产品中丙硫唑的残留分析。

英文摘要:

      Objective To establish a method for determination of albendazole residues in 8 kinds of agriculture products by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods Based on the classical QuEChERS technique, the extraction agent and purifying agent of the pretreatment method were optimized. The samples were extracted with acetonitrile containing 1% (V:V) acetic acid and purified with octadecylsilane, and external standard method was used for quantification. Results The concentration of albendazole had good linear relationships with the peak area in the range of 0.00025?0.50 mg/L with the correlation coefficients all greater than 0.99. The average recoveries were in the range of 70%?110% and the relative standard deviations were in the range of 0.83%?10.65% at 0.001, 0.01 and 0.1 mg/kg supplemental levels, the limits of quantitation (LOQ) and limits of detection (LOD) of albendazole in 8 kinds of agriculture products samples were 0.001 mg/kg. Conclusion The method has the advantages of rapid, simple, low cost and high accuracy, etc. It is suitable for the determination of albendazole residues in 8 kinds of agricultural products of rice, tobacco, pepper, watermelon, cabbage, wheat, grape and banana.