重组酶介导扩增技术快速检测水产品中副溶血性弧菌

Rapid detection of Vibrio parahaemolyticus in aquatic products by recombinase aid amplification

中文关键词:  副溶血性弧菌  重组酶介导扩增技术  快速检测

英文关键词:Vibrio parahaemolyticus  recombinase aid amplification  rapid detection

中文摘要:

      目的 建立重组酶介导扩增技术(recombinase aid amplification, RAA)快速检测副溶血性弧菌的分析方法。方法 根据副溶血性弧菌(Vibrio parahaemolyticus) tlh基因序列设计特异性引物, 经引物筛选, 建立副溶血性弧菌的RAA的快速检测方法。结果 该方法在30 min即可观察到检测结果。特异性试验结果表明RAA方法检测副溶血性弧菌与其他弧菌属菌种不存在交叉反应, 灵敏度试验分析结果表明RAA方法检测副溶血性弧菌检出限为5.3 CFU/mL。用100份样品验证RAA方法的可靠性, 结果显示RAA方法和传统划线方法结果符合率为100%, 表现出高度一致性。结论 本研究建立的RAA检测方法具有特异性强、灵敏度高、操作简便、快速等优点, 可用于水产品中副溶血性弧菌的检测。

英文摘要:

      Objective To establish a method for detection of Vibrio parahaemolyticus by recombinase aided amplification (RAA). Methods Based on the tlh gene sequence of Vibrio parahaemolyticus, the specific primers were designed. After screening the primers, a rapid detection method of RAA was established. Results The detection results could be observed within 30 min. The results of specificity test showed that there was no cross reaction between the detection of Vibrio parahaemolyticus by RAA method and other Vibrio species. The results of sensitivity test showed that the limit of detection Vibrio parahaemolyticus by RAA method was 5.3 CFU/mL. A total of 100 samples were used to verify the reliability of the RAA method. The results show that the coincidence rate of the RAA method and the traditional crossing method was 100%, showed a high degree of consistency. Conclusion The RAA detection method established in this study has the advantages of strong specificity, high sensitivity, simple operation and rapidity, and can be used for the detection of Vibrio parahaemolyticus in aquatic products.