冷等离子体处理去除大豆油中黄曲霉毒素B1的研究

Study on the removal of aflatoxin B1 in soybean oil by cold plasma treatment

中文关键词:  冷等离子体  大豆油  黄曲霉毒素B1  脱除

英文关键词:cold plasma  soybean oil  aflatoxin B1  removal

中文摘要:

      目的 探究冷等离子体技术对大豆油中的黄曲霉毒素B1 (aflatoxin B1, AFB1)的脱除影响因素。方法 采用高效液相色谱法(high performance liquid chromatography, HPLC)分析冷等离子体技术对AFB1的脱除效果, 通过单因素和响应面试验设计研究不同处理电压、处理时间和pH对AFB1的消减情况。结果 根据AFB1浓度和峰面积建立标准工作曲线, 得到曲线方程为Y=4396.9X?1707.6, r2=0.9997。通过响应面优化可知, 冷等离子体技术脱除大豆油中AFB1的最适条件为: 处理时间95.08 s、处理功率143.30 W、pH为8.89, 在初始浓度为(95.56±3.53) μg/mL时, 此条件下冷等离子体处理后, 大豆油中AFB1的浓度为(21.73±1.68) μg/mL, 脱除率为(79.65±1.33)%, 分别与预测值22.49 μg/mL、80.12%的吻合度达96%和99%, 说明该模型优化较为合理。 结论 冷等离子体技术对大豆油中的AFB1具有良好的脱除效果, 冷等离子体作为非热加工技术脱除真菌毒素方向有广阔的应用前景。

英文摘要:

      Objective To investigate the effect of cold plasma technology on the removal of aflatoxin B1 (AFB1) from soybean oil. Methods The effect of cold plasma technology on the removal of AFB1 was analyzed by high performance liquid chromatography (HPLC), and the effect of different treatment voltages, treatment times and pH on the reduction of AFB1 were investigated by single-factor and response surface tests. Results The standard working curve was established based on the concentration and peak area of AFB1, and the equation of the curve was Y=4396.9X?1707.6, r2=0.9997. The optimum conditions for the removal of AFB1 from soybean oil by cold plasma technology were 95.08 s, 143.30 W and pH 8.89. At an initial concentration of (95.56±3.53) μg/mL, the concentration of AFB1 in soybean oil was (21.73±1.68) μg/mL and the removal rate was (79.65±1.33)% after cold plasma measurement under above condition. The model optimization was reasonable, as the results were 96% and 99% consistent with the predicted values of 22.49 μg/mL and 80.12% respectively. Conclusions Cold plasma technology has good effect on the removal of AFB1 from soybean oil, and it has a promising application as a non-thermal processing technology for the removal of fungal toxins.