红托竹荪多糖的提取优化及膜分级分离的研究

Study on optimization of extraction and membrane fractionation of polysaccharide from Dictyophora rubrovolvata

中文关键词:  红托竹荪  多糖  超声  响应面优化  膜分离

英文关键词:Dictyophora rubrovolvata  polysaccharide  ultrasonic  response surface methodology  membrane separation

中文摘要:

      目的 研究红托竹荪多糖的超声辅助提取工艺及其膜分级分离方法。方法 样品采用超声辅助方法提取, 以多糖提取率为指标, 通过单因素试验结合响应面优化得到红托竹荪多糖的最佳提取工艺参数, 并采用5种不同截留分子量的滤膜对红托竹荪粗多糖进行分级分离, 比较这5种滤膜对红托竹荪粗多糖的分级效果。结果 红托竹荪多糖的最佳提取工艺参数为: 超声功率480 W, 超声时间35 min, 提取次数2次, 料液比1:20 (g/mL), 在此条件下多糖提取率为(14.55±0.16)%, 多糖含量为(60.33±1.53)%。膜分离中, 截留分子量为100 kDa的超滤膜所分离的多糖占比最大, 多糖含量提升至(90.87±2.57)%, 多糖保留率为95.68%。结论 超声辅助提取工艺合理可行, 膜分离技术可有效提高多糖纯度, 为红托竹荪多糖工业化生产提供理论基础。

英文摘要:

      Objective To study the ultrasonic-assisted extraction process and membrane fractionation method of polysaccharide from Dictyophora rubrovolvata. Methods Samples were extracted by ultrasound-assisted method. Taking extraction rate of polysaccharide as index, the optimal extraction parameters were obtained by single factor experiment combined with response surface optimization. In addition, 5 different kinds of filter membranes with different molecular weight cut-off were used to separate the crude polysaccharide of Dictyophora rubrovolvata, and the classification effects of these 5 kinds of filter membranes on the crude polysaccharide of Dictyophora rubrovolvata were compared. Results The optimal extraction parameters of Dictyophora rubrovolvata polysaccharide were as follows: Ultrasonic power was 480 W, ultrasonic time was 35 min, extraction times was twice, solid-liquid ratio was 1:20 (g/mL). Under these conditions, the extraction yield of polysaccharide was (14.55±0.16)%, and the content of polysaccharide was (60.33±1.53)%. In the membrane separation, the proportion of polysaccharide separated by ultrafiltration membrane with molecular weight cut-off of 100 kDa was the largest, the polysaccharide content increased to (90.87±2.57)%, and the retention rate of polysaccharide was 95.68%. Conclusion Ultrasonic assisted extraction technology is reasonable and feasible, and membrane separation technology can effectively improve the purity of polysaccharide, which provides a theoretical basis for the industrial production of polysaccharide from Dictyophora rubrovolvata.