益生菌冻干保护剂及工艺优化研究

Freeze-drying protective agent and its process optimization of probiotics

中文关键词:  冻干保护剂  益生菌  工艺优化

英文关键词:freeze-drying protective agent  probiotics  process optimization

中文摘要:

      目的 探究益生菌冻干保护剂对其存活率的影响, 并优化其工艺。方法 采用真空冷冻干燥法, 以保加利亚乳杆菌为研究对象, 探究冻干保护剂对益生菌冻干存活率的影响, 并通过单因素和正交试验筛选出最优组合, 优化其工艺。结果 经-80 ℃预冻1 h, 冷冻干燥48 h (?40 ℃、25 Pa)后, 益生菌保加利亚乳杆菌的最佳冻干保护剂为甘油2%, 脱脂乳6%, L-半胱氨酸3%, 木聚糖10%, 在此优化条件下, 存活率为82.75%。 结论 在冷冻干燥过程中加入冻干保护剂可以提高保加利亚乳杆菌存活率。

英文摘要:

      Objective To explore the effect of freeze-drying protective agent on the survival rate of probiotics, and optimize the process. Methods Vacuum freeze-drying method was used to explore the effect of protective agent on the survival rate of probiotics by taking Lactobacillus bulgaricus as the research object, and the optimal combination was selected through single factor and orthogonal tests to optimize the process. Results After pre-freezing at -80 ℃ for 1 h and freeze-drying for 48 h (?40 ℃, 25 Pa), the optimal freeze-drying protective agent of Lactobacillus bulgaricus was 2% glycerin, 6% skim milk, 3% L-cysteine and 10% xylan. Under the optimized conditions, the survival rate was 82.75%. Conclusion The survival rate of Lactobacillus bulgaricus can be improved by adding freeze-drying protective agent in the freeze-drying process.