肉牛唾液作为氯丙那林残留易获表征样本的可行性研究

Study on the feasibility of beef cattle saliva as an easily available characterization sample of clorprenaline residue

中文关键词:  肉牛唾液  氯丙那林  残留  易获表征样本  可行性研究

英文关键词:beef cattle saliva  clorprenaline  residual  easily available characterization sample  feasibility study

中文摘要:

      目的 评估肉牛唾液作为氯丙那林(clorprenaline, CLO)残留易获表征样本的可行性。方法 选取4只雌性健康育肥期肉牛(500±25) kg, 其中1只作为对照, 另3只作为实验组, 每日将氯丙那林按1 mg/(kg·bw)经基础日粮混饲实验组肉牛1次, 连续给药25 d。基于高效液相色谱-串联质谱技术(high performance liquid chromatography-tandem mass spectrometry, HPLC-MS/MS), 建立肉牛唾液中的氯丙那林残留量检测方法, 并对给药期内肉牛唾液中的氯丙那林残留量进行跟踪测定。结果 供试品溶液中氯丙那林浓度在0.1~100.0 ng/mL范围内时, 浓度-峰面积线性关系良好; 检出限为1.0 ng/mL; 方法回收率在73.7%~87.2%之间, 重现性良好。混饲给药24 h后, 3头受试肉牛唾液中氯丙那林残留量在33.5~119 ng/mL; 连续给药25 d的期间内, 尽管残留量表现出较大的个体差异, 但受试牛唾液中氯丙那林残留量均呈逐步上升趋势, 最高值达到273.6 ng/mL。 结论 肉牛唾液较血液和尿液易于获取, 基于高效液相色谱串联-质谱法, 能准确地反映养殖过程非法使用氯丙那林的情况, 可作为行政监管的易获表征组织。

英文摘要:

      Objective To evaluate the feasibility of beef cattle saliva as an easily available characterization sample of clorprenaline (CLO) residue. Methods Four female healthy fattening cattle (500±25) kg were selected, of which one was used as the control and the other three were used as the experimental group. The beef cattle in the experimental group were mixed with CLO at 1 mg/(kg·bw) per day through the basic diet, and the treatment was given continuously for 25 days. A method for the determination of CLO residue in beef saliva was established based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technology, and the CLO residues in beef saliva during the dosing period were tracked and determined. Results Based on HPLC-MS/MS method: When CLO levels in the final test solution were between 0.1 ng/mL and 100.0 ng/mL, the linear relationship was good; the limit of quantitation was 1.0 ng/mL and the recoveries rate were between 73.7% and 87.2%. After oral administration in 24 hours, the residual amount of CLO in saliva of 3 tested beef cattle ranged from 33.5 ng/mL to 119 ng/mL; although the residual levels showed great individual differences, during 25 days of continuous administration, the residual amount of CLO in saliva of tested cattle showed a gradual upward trend, with the highest value reaching 273.6 ng/mL. Conclusion Based on HPLC-MS/MS method, the saliva of cattles, which is easier to obtain than blood and urine, can accurately reflect the illegal use of CLO in breeding. Therefor it can be used as an easily available characterization organization for administrative supervision.