超高效液相色谱-串联质谱法测定果蔬中28种植物生长调节剂残留量

Determination of 28 kinds of plant growth regulators residues in fruits and vegetables by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  超高效液相色谱-串联质谱法  植物生长调节剂  果蔬  MPFC-QuEChERS超滤净化柱

英文关键词:ultra performance liquid chromatography-tandem mass spectrometry  plant growth regulators  fruits and vegetables  MPFC-QuEChERS ultrafiltration column

中文摘要:

      目的 建立超高效液相色谱-串联质谱法快速测定果蔬中28种植物生长调节剂(plant growth regulators, PGRs)残留量的方法。方法 样品经1%甲酸乙腈提取, QuEChERS方法净化后, 根据样品状态, 最后再经过MPFC-QuEChERS超滤净化柱进行滤过式净化。采用5 mmol/L乙酸铵0.1%甲酸水溶液-乙腈梯度洗脱, Hypersil GOLD柱进行分离, 多反应监测模式监测, 用上海青、豆芽、苹果3种基质分别匹配标准曲线, 外标法定量。结果 各组分在20~300 ng/mL范围内线性关系良好, r≥0.990。在4、20、60 μg/kg 3个不同加标浓度下, 平均回收率为60.49%~120.96%, 相对标准偏差为0.3%~14.6%。定量限为0.09~4.06 μg/kg。结论 该方法快速、准确、干扰小, 可满足实验室对于常见类别果蔬的植物生长调节剂进行快速筛查。

英文摘要:

      Objective To establish a method for the determination of 28 kinds of plant growth regulators (PGRs) in fruits and vegetables by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods Samples were extracted with 1% formic acid acetonitrile and purified by QuEChERS method, and finally filtered through the MPFC-QuEChERS ultrafiltration purification column according to the state of the sample. The targets were separated with a Hypersil GOLD column using 5 mmol/L ammonium acetate solution contain 0.1% formic acid and acetonitrile as the mobile phases in gradient program, and then analyzed by multiple reaction monitoring mode (MRM). Three matrixes including Shanghai cabbage, bean sprout and apple were matched with the standard curve and the content was quantified by external standard method. Results Each component had a good linear relationship in the range of 20?300 ng/mL, r≥0.990. At 3 different spiked concentrations of 4, 20 and 60 μg/kg, the average recoveries were 60.49%?120.96% and the relative standard deviations were 0.3%?14.6%. The limits of quantitation were 0.09?4.06 μg/kg. Conclusion The method is fast, accurate and had lower interference, which can be used for laboratory rapid screening of plant growth regulators in common fruits and vegetables.