基于转录组的氯吡脲对草莓果实成熟的调控机制研究

Transcriptome-based to study the regulation mechanism of forchlorfenuron on strawberry fruit ripening

中文关键词:  氯吡脲  草莓  转录组学  差异基因  机制

英文关键词:forchlorfenuron  strawberry  transcriptomics  differential gene  mechanism

中文摘要:

      目的 通过对对照组和白熟期外源施用氯吡脲处理组的成熟草莓果实进行转录组学分析, 探讨外源喷施氯吡脲影响草莓果实成熟的调控机制。方法 利用高通量测序技术RNA-Seq, 结合生物信息学方法, 进行差异基因的筛选和其通路的功能富集分析。结果 草莓白熟期喷施氯吡脲, 成熟果实中有1176个基因表达上调, 2031个基因表达下调。差异基因的富集分析发现, 植物-病原体相互作用、植物激素信号传导、淀粉和蔗糖代谢、内质网蛋白加工以及次级代谢物的生物合成等通路为差异基因主要富集的代谢途径。结论 植物激素信号转导、植物-病原体相互作用和次级代谢物的生物合成等通路受氯吡脲影响最为显著, 其中植物激素信号转导通路是通过不同激素的共同调控来影响草莓的生长成熟。研究结果为氯吡脲影响草莓果实成熟调控基因的挖掘和深入研究奠定了基础。

英文摘要:

      Objective To analyze the transcriptomics analysis of the mature strawberry fruits of the control group and the treatment group treated with exogenous forchlorfenuron during the white maturity period, and explore the regulatory mechanism of exogenous spraying of forchlorfenuron on strawberry fruit ripening. Methods The differential genes were screened and the functional enrichment of their pathways were analyzed by high-throughput sequencing technology RNA-Seq and bioinformatics methods. Results Totally 1176 genes were up-regulated and 2031 genes were down regulated in the mature strawberry fruits sprayed with chlorfenuron at white ripening stage. Enrichment analysis of differentially expressed genes revealed that plant pathogen interaction, plant hormone signaling, starch and sucrose metabolism, endoplasmic reticulum protein processing and secondary metabolite biosynthesis were the main metabolic pathways of differentially expressed genes. Conclusion Plant hormone signal transduction pathway, plant pathogen interaction pathway and secondary metabolite biosynthesis pathway are most significantly affected by chlorfenuron, and plant hormone signal transduction pathway affects strawberry growth and maturity through the joint regulation of different hormones. The results lays a foundation for the further research and mining of genes regulating the effect of chlorfenuron on strawberry fruit ripening.