SB/T 10923—2012掺假肉类DNA检测方法的改进与评价

Improved and evaluation of SB/T 10923—2012 DNA detection method for adulterated meat

中文关键词:  实时荧光PCR  猪肉  牛肉  羊肉  鸡肉  鸭肉

英文关键词:real-time PCR  pork  beef  sheep  chicken  duck

中文摘要:

      目的 对SB/T 10923—2012《肉及肉制品中动物源性成分的测定 实时荧光PCR法》检测方法进行改进, 评价其应用于掺假肉DNA鉴定的价值。方法 评价改进后方法的特异性和掺假比例检测能力, 并用7份预包装生鲜冷冻肉制品考察实际检验效能。结果 改进后的方法在Cq阈值为30时, 能够有效区分20 ng/反应的特异模板, 最低可检出0.5% (w:w)的掺假比例。对7份样品共进行24个肉源成分检测, 与标签符合率为95.8% (23/24)。有1种鸭源性成分未检出, 可能和混样不均匀有关。结论 改进后的方法可快速、特异、简便地用于掺假肉常规检测。

英文摘要:

      Objective To evaluate the value of the improved SB/T 10923—2012 Identification of animal derived materials in meat and meat products-real-time PCR methods in DNA detection of adulteration meat. Methods The specificity and adulteration ratio detection ability of the improved method were evaluated, and the actual inspection efficiency was investigated with 7 pre-packaged fresh frozen meat products. Results When the Cq threshold was 30, the improved method could effectively distinguish the specific template at 20 ng/reaction, and the lowest detectable ratio of adulteration was 0.5% (w:w). A total of 24 meat-derived components were detected from 7 samples, and the coincidence rate with the label was 95.8% (23/24). One duck-derived component was not detected, which might be related to uneven sample mixing. Conclusion The improved method could be rapidly, specifically and conveniently used for routine detection of adulterated meat.