基于网络药理学分析红芪免疫调节的作用机制

Mechanism of Hedysari radix on immunoregulation based on network pharmacology

中文关键词:  红芪  网络药理学  免疫调节  机制

英文关键词:Hedysari radix  network pharmacology  immunoregulation  mechanism

中文摘要:

      目的 基于网络药理学方法探讨红芪免疫调节的物质基础及作用机制。方法 借助中药系统药理学(traditional Chinese medicine systems pharmacology, TCMSP)数据库与分析平台检索红芪的化学成分和相关作用靶点。进而采用Cytoscape 3.6.1、STRING 11.0软件构建化合物-靶点网络和蛋白互作(protein-protein interaction, PPI)网络, 运用DAVID 6.7在线数据库进行基因本体(gene ontology, GO)功能富集分析和基于京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes, KEGG)生物通路富集分析, 研究红芪对免疫调节的作用机制。结果 通过TCMSP检索到43个化合物, 筛选得出20个活性化合物, 预测潜在靶点225个。PPI网络包含36个靶点, 基因本体条目27个, 其中生物过程相关条目22个, 分子功能相关条目3个, 细胞组成相关条目2个。京都基因与基因组百科全书通路8条。结论 红芪中多种化学成分可能通过与PTGS、PRKACA、ADRB2、RXRA、HSP90等靶点结合, 从而发挥免疫调节作用。红芪对免疫调节具有多成分、多靶点、多途径的特点, 对心脑血管疾病、炎症、癌症等具有潜在的治疗作用, 研究结果可为红芪对免疫调节作用机制提供一定的理论基础与科学依据。

英文摘要:

      Objective To study material basis and mechanism of Hedysari radix on immunoregulation based on network pharmacology. Methods The chemical components and corresponding targets related of Hedysari radix were searched from the traditional Chinese medicine systems pharmacology (TCMSP) database and analysis platform. The compound-target network and the protein-protein interaction (PPI) network were established by Cytoscape 3.6.1 and STRING 11.0 software, and the gene ontology (GO) functional enrichment analysis and the Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were made by DAVID 6.7 software to explore the mechanism of Hedysari radix. Results Through TCMSP, 43 kinds of compounds were identified, 20 kinds of active compounds were screened and 225 kinds of potential targets were predicted. The PPI network contains 36 kinds of targets, and there were 27 kinds of GO entries, including 22 kinds of biological process entries, 3 kinds of molecular function entries and 2 kinds of cell composition related items. Besides, there were 8 kinds of KEGG pathways. Conclusion Several chemical components of Hedysari radix may be combined with PTGS, PRKACA, ADRB2, RXRA, HSP90 and so on to play an immunomodulatory role. Hedysari radix has the characteristics of multiple components, multiple targets and multiple pathways for immunoregulation, and has potential therapeutic effects on cardiac cerebrovascular disease, inflammation, cancer, etc. The results of this study may provide a theoretical basis and scientific basis for the mechanism of immune regulation of Hedysari radix.