高效液相色谱法测定速溶滇红茶制品中茶黄素含量

Determination of theaflavins in instant Yunnan black tea products by high performance liquid chromatography

中文关键词:  高效液相色谱法  滇红茶  速溶茶  茶黄素

英文关键词:high performance liquid chromatography  Yunnan black tea  instant tea  theaflavins

中文摘要:

      目的 建立高效液相色谱法测定速溶滇红茶制品中茶黄素含量的分析方法。方法 样品提取后, 采用Agilent ZORBAX SB-C18色谱柱分离, 以0.1%甲酸乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱, 流速为 1 mL/min, 柱温30 ℃, 检测波长为280 nm, 外标法定量。结果 4种茶黄素单体在各自浓度范围内线性关系良好, 相关系数均大于0.9995, 检出限为1.1~1.6 μg/g, 定量限为3.7~5.5 μg/g, 加标回收率为95.08%~104.50%, 相对标准偏差均小于2.01%。结论 本检测方法具有较好的重复性、精密度及稳定性, 适用于检测速溶滇红茶制品中茶黄素的含量。

英文摘要:

      Objective To establish a method for the determination of theaflavins in instant Yunnan black tea products by high performance liquid chromatography. Methods After extraction, the samples were separated by Agilent ZORBAX SB-C18column using 0.1% formic acid acetonitrile-0.1% formic acid aqueous solution as the mobile phase for gradient elution. The flow rate was 1 mL/min, the column temperature was 30 ℃, and the detection wavelength was 280 nm. The samples were quantified by external standard method. Results The 4 theaflavins had good linear relationships within their respective concentration ranges, the correlation coefficients were all greater than 0.9995, the limits of detection were 1.1?1.6 μg/g, the limits of quantification were 3.7?5.5 μg/g, the standard recoveries were 95.08%?104.50%, and the relative standard deviations were less than 2.01%. Conclusion This method has good repeatability, precision and stability, and is suitable for the determination of theaflavins in Yunnan black instant tea products.