高效液相色谱-高分辨质谱法快速测定猪肉中的阿托品和普鲁卡因残留

Rapid determination of atropine and procaine residues in pork by high performance liquid chromatography-high resolution mass spectrometry

中文关键词:  阿托品  普鲁卡因  猪肉  残留  高效液相色谱-高分辨质谱法

英文关键词:atropine  procaine  pork  residues  high performance liquid chromatography-high resolution mass spectrometry

中文摘要:

      目的 建立高效液相色谱-高分辨质谱法测定猪肉中阿托品和普鲁卡因残留的分析方法。方法 样品经乙腈提取, 采用通过式吸附净化柱净化, 高效液相色谱C18柱分离化合物, 高分辨质谱分析测定, 平行反应监测模式定量检测, 外标法定量。结果 阿托品和普鲁卡因保留时间分别为5和4 min, 在0.1~5.0 μg/kg之间线性关系良好, 批内、批间精密度均小于20%, 回收率稳定在85%~105%之间, 定量限为0.1 μg/kg。结论 该方法灵敏、快速、准确度高, 可用于监测猪肉中2种药物残留, 可为畜产品质量安全保障提供高效的技术支撑。

英文摘要:

      Objective To establish an analytical method for determination of atropine and procaine residues in pork by high performance liquid chromatography-high resolution mass spectrometry. Methods The samples were extracted with acetonitrile, purified by a mobile adsorption purification column, and the compounds were separated on a high performance liquid chromatography C18 column, analyzed and determined by high-resolution mass spectrometry, detected quantitatively in parallel reaction monitoring mode, and quantified by external standard method. Results The retention times of atropine and procaine were 5 and 4 min, respectively. The linear relationships were good within the range of 0.1-5.0 μg/kg. The intra-assay and inter-assay precision were less than 20%, the recoveries were stable within the range of 85%-105%, and the limits of quantification were 0.1 μg/kg. Conclusion This method is sensitive, rapid and accurate, and can be used to monitor the residues of 2 kinds of drugs in pork, which can provide efficient technical support for the quality and safety assurance of livestock products.