衍生化-富集-表面增强拉曼光谱法快速筛查奶粉中亚硝酸盐

Rapid detection of nitrite in milk powder by derivatization- enrichment-surface enhanced Raman spectroscopy

中文关键词:  表面增强拉曼光谱法  衍生化  富集  奶粉  亚硝酸盐  快速筛查

英文关键词:surface enhanced Raman spectroscopy  derivatization  enrichment  milk powder  nitrite  rapid detection

中文摘要:

      目的 建立衍生化-富集-表面增强拉曼光谱法快速检测奶粉中亚硝酸盐的分析方法。方法 样品水溶液通过除蛋白、衍生化后, 采用阳离子小柱PSY-001对衍生化合物进行富集, 再使用表面增强拉曼光谱对样品中的亚硝酸盐含量进行定性检测。结果 7类不同奶粉, 采用本研究所建立的方法进行检测, 根据不同的批处理量, 单个样品检测全过程只需3~15 min, 定性检测限为0.4 mg/kg, 远低于国家安全标准对亚硝酸盐的限量要求(2 mg/kg)。方法的灵敏度在96%～100%, 特异性为100%, 假阴性率为0%～4%, 假阳性率为0%, 相对准确度为98%～100%。结论 该方法操作简单、快速, 易于掌握, 适用于奶粉中亚硝酸盐的快速筛查。

英文摘要:

      Objective To eatablish a method for the rapid detection of nitrite in milk powder by derivatization-enrichment-surface enhanced Raman spectroscopy. Methods The aqueous solutions of samples were deproteinized and derivatized, and the derived compounds were enriched by cationic cartridge PSY-01, finally, the nitrite in samples were qualitative detected by surface enhanced Raman spectroscopy. Results Seven kinds of milk powders were tested by this method, which took only 3?15 minutes per sample, depending on batch processing capacity. The limits of detection (LOD) of this method was 0.4 mg/kg and much less than maximum residue limit (MRL) of nitrite specified in national food safety standard of China (2 mg/kg). The sensitivity of this method was 96%?100%, the specificity was 100%, the false negative rate was 0%?4%, the false positive rate was 0%, and the relative accuracy was 98%?100%. Conclusion This method is simple, quick and easy to operate, and therefore suitable for the rapid screening of nitrite in milk powder.