食物中毒事件中致病菌肠毒素检测和分子分型研究

Molecular typing and enterotoxin detection in food poisoning case

中文关键词:  金黄色葡萄球菌  食物中毒  葡萄球菌肠毒素  脉冲场凝胶电泳

英文关键词:Staphylococcus aureus  food poisoning  staphylococcal enterotoxin  pulsed field gel electrophoresis

中文摘要:

      目的 为了检测一起食物中毒事件样本, 并进行相关性分析、分离菌株。方法 参照国家标准方法GB 4789系列, 对采集样本进行细菌学检验。应用酶联免疫吸附法(enzyme-linked immunosorbent assay, ELISA)检测葡萄球菌肠毒素。通过脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)进行分子分型, 分析菌株之间的相关性。结果 食物中毒事件中5名患者均检测出E型葡萄球菌肠毒素(staphylococcal enterotoxin E, SEE)。3株分离菌株PFGE分子分型提示来源不同克隆株, 除了产生E型葡萄球菌肠毒素外, 还有B、C、D型。结论 该起食物中毒由不同PFGE型产毒金黄色葡萄球菌污染食物引起, 菌株产葡萄球菌肠毒素的型别不完全相同。

英文摘要:

      Objective To detect a food poisoning event samples, and conduct correlation analysis, isolate the strain . Methods According to the national standard methods GB 4789 series, the collected samples were tested for bacteriology. Enzyme-linked immunosorbent assay (ELISA) was used to detect staphylococcal enterotoxin. Molecular typing was performed by pulsed field gel electrophoresis (PFGE) to analyze the correlation between strains. Results Staphylococcal enterotoxin E (SEE) was detected in all 5 cases of food poisoning. The PFGE molecular typing of the 3 isolates indicated that the clones from different sources produced types B, C and D besides E. Conclusion The food poisoning is caused by contamination of food by different strains of PFGE toxigenic Staphylococcus aureus, with different types of staphylococcal enterotoxin.