液相色谱-线性离子阱-串联质谱法测定海参中127种兽药残留

Determination of 127 kinds of veterinary drugs in sea cucumber by liquid chromatography-Qtrap-tandem mass spectrometry

中文关键词:  兽药残留  线性离子阱  液相色谱-串联质谱法  海参

英文关键词:veterinary drug residue  linear ion trap  liquid chromatography-tandem mass spectrometry  sea cucumber

中文摘要:

      目的 建立液相色谱-线性离子阱-串联质谱法测定海参中127种兽药残留量的分析方法。方法 样品经乙腈:水:甲酸(75:25:0.1, V:V:V)提取, 涡旋振荡5 min后超声提取30 min, 9000 r/min离心10 min, 滤膜过滤后采用液相色谱-质谱联用仪测定。结果 该方法检出限可达到5 μg/kg, 127种兽药均在9 min之内流出, 线性相关系数大于0.99, 回收率在30%~116.2%之间, 相对标准偏差在15%以内。结论 与传统的检测方法相比, 该方法样品制备简单, 仪器检测速度快, 具有很好的实用性。

英文摘要:

      Objective To establish a method for the determination of 127 kinds of veterinary drug residues in sea cucumbers by liquid chromatography-Qtrap-tandem mass spectrometry. Methods The sample was extracted by acetonitrile:water:formic acid (75:25:0.1, V:V:V), vortex oscillation for 5 min, ultrasonic extraction for 30 min, centrifugation at 9000 r/min for 10 min, filtration by membrane, and determined by liquid chromatography-tandem mass spectrometry. Results The detection limit of this method could reach 5 μg/kg, 127 kinds of veterinary drugs could flow out within 9 min, the linear correlation coefficient was greater than 0.99, the recoveries were between 30%?116.2%, and the relative standard deviation was lower than 15%. Conclusion Compared with the traditional detection, this method provides an alternative with easier sample preparation, faster instrumental detection and has good practicability.