气相色谱-质谱联用法分析牛尾菜根和茎叶中挥发性成分的差异

Difference analysis of volatile components in roots, stems and leaves of Smilax riparia A.DC by gas chromatography-mass spectrometry

中文关键词:  牛尾菜  挥发油  气相色谱-质谱联用法  根  茎叶

英文关键词:Smilax riparia A.DC  volatile oil  gas chromatography-mass spectrometry  root  stems and leaves

中文摘要:

      目的 分析牛尾菜根和茎叶中的挥发油成分。方法 采用甲醇超声提取, 乙酸乙酯萃取法提取挥发油。然后采用气相色谱-质谱联用法(gas chromatography-mass spectrometry, GC-MS)进样分析获得总离子流图, 采用Xcalibur对总离子流图中各色谱峰进行质谱扫描后, 经过质谱计算机数据系统检索NIST图谱库鉴定牛尾菜根茎叶中挥油的化学成分, 并采用峰面积归一化法计算各成分的相对质量分数。结果 牛尾菜根挥发油鉴定出25种成分, 主要成分为乙酸丁酯、9C,11TR-共轭亚油酸甲酯、邻苯二甲酸二丁酯等; 牛尾菜茎挥发油鉴定出36种成分, 主要成分为邻苯二甲酸正庚-4-基酯、辛那普利醇等; 牛尾菜叶挥发油鉴定出29种成分, 主要成分为邻苯二甲酸二丁酯、2,2'-亚甲基双-(4-甲基-6-叔丁基苯酚)等, 3种挥发油共鉴定出72种成分。结论 本研究基本明确了牛尾菜根和茎叶挥发油中的主要成分, 并且发现三者存在差异。

英文摘要:

      Objective To analyze the chemical differences between volatile oils extracted from the roots, stems and leaves of Smilax riparia A.DC. Methods The volatile oil was extracted by methanol ultrasonic. Gas chromatography-mass spectrometry (GC-MS) was adopted to analyze volatile oil to obtain total ion chromatogram (TIC). After mass spectra scanning of the chromatographic peaks in the TIC diagram by Xcalibur, chemical components of volatile oil in Smilax riparia A.DC were identified by retrieving and comparing mass spectrum database. The peak area normalization method was used to calculate the relative mass fraction of each component. Results After the obtained data were analyzed, 25 kinds of components were identified from the volatile oil of the root of the Smilax riparia A.DC, main components were butyl acetate, 9C,11TR conjugated linoleic acid methyl ester, and dibutyl phthalate. Totally 36 kinds of components were identified from the volatile oil of the stems of the Smilax riparia A.DC, main components were n-heptyl-4-yl phthalate and trans-sinapyl alcohol, and 29 kinds of components were identified from the volatile oil of the leaves of the Smilax riparia A.DC, main components were dibutyl phthalate and 2,2'-methylene bis-(4-methyl-6-tert-butylphenol). A total of 72 components were identified from 3 kinds of volatile oils. Conclusion In this study, the main components of the volatile oil in the roots, stems and leave of the Smilax riparia A.DC are basically identified, and there are differences among them.